open access

Vol 49, No 4 (2011)
ORIGINAL PAPERS
Published online: 2012-01-16
Submitted: 2012-01-16
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Localization of substance P, calcitonin gene related peptide and galanin in the nerve fibers of porcine cystic ovaries

Anna Kozłowska, Joanna Wojtkiewicz, Mariusz Majewski, Barbara Jana
DOI: 10.5603/FHC.2011.0085
·
Folia Histochem Cytobiol 2011;49(4):622-630.

open access

Vol 49, No 4 (2011)
ORIGINAL PAPERS
Published online: 2012-01-16
Submitted: 2012-01-16

Abstract

In a previous study, we showed that both the noradrenergic and cholinergic component of ovarian innervation is markedly changed in porcine cystic ovaries. The present study is aimed at elucidating the distribution pattern of substance P- (SP), calcitonin gene related peptide CGRP- and/or galanin (GAL)-containing nerve fibers within porcine cystic ovaries. The status polycysticus was induced by dexamethasone phosphate disodium salt i.m. injections performed from the 7th until the 21st day of the first studied estrous cycle. During the same period of time, gilts of the control group received saline. All animals were slaughtered on the expected 11th day of the second studied estrous cycle, and their ovaries were collected. When compared to control gonad, a distinct difference in the distribution pattern and the density of SP-, CGRP- and/or GAL-immunoreactive (GAL-IR) nerve fibers was observed. Thus, unlike in the control gonad, SP- and/or CGRP-IR perivascular nerve fibers were found to supply medullar blood vessels of polycystic ovary. Furthermore, the number of GAL-IR nerve fibers contributing to the ground plexus in polycystic ovaries was higher than that observed in the control gonads. Thus, as may be judged from the profound changes in the distribution pattern of differently chemically coded afferent terminals within polycystic gonads, it appears possible that neuropeptides released from these terminals may take part in the etiopathogenesis of this disorder. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 4, pp. 622–630)

Abstract

In a previous study, we showed that both the noradrenergic and cholinergic component of ovarian innervation is markedly changed in porcine cystic ovaries. The present study is aimed at elucidating the distribution pattern of substance P- (SP), calcitonin gene related peptide CGRP- and/or galanin (GAL)-containing nerve fibers within porcine cystic ovaries. The status polycysticus was induced by dexamethasone phosphate disodium salt i.m. injections performed from the 7th until the 21st day of the first studied estrous cycle. During the same period of time, gilts of the control group received saline. All animals were slaughtered on the expected 11th day of the second studied estrous cycle, and their ovaries were collected. When compared to control gonad, a distinct difference in the distribution pattern and the density of SP-, CGRP- and/or GAL-immunoreactive (GAL-IR) nerve fibers was observed. Thus, unlike in the control gonad, SP- and/or CGRP-IR perivascular nerve fibers were found to supply medullar blood vessels of polycystic ovary. Furthermore, the number of GAL-IR nerve fibers contributing to the ground plexus in polycystic ovaries was higher than that observed in the control gonads. Thus, as may be judged from the profound changes in the distribution pattern of differently chemically coded afferent terminals within polycystic gonads, it appears possible that neuropeptides released from these terminals may take part in the etiopathogenesis of this disorder. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 4, pp. 622–630)
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Keywords

polycystic ovaries; sensory innervation; neuropeptides; nerve fibers; ovarian cysts; gilts

About this article
Title

Localization of substance P, calcitonin gene related peptide and galanin in the nerve fibers of porcine cystic ovaries

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 49, No 4 (2011)

Pages

622-630

Published online

2012-01-16

DOI

10.5603/FHC.2011.0085

Bibliographic record

Folia Histochem Cytobiol 2011;49(4):622-630.

Keywords

polycystic ovaries
sensory innervation
neuropeptides
nerve fibers
ovarian cysts
gilts

Authors

Anna Kozłowska
Joanna Wojtkiewicz
Mariusz Majewski
Barbara Jana

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