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Vol 49, No 4 (2011)
Original paper
Submitted: 2012-01-16
Published online: 2012-01-16
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No correlation between pinopode formation and LIF and MMP2 expression in endometrium during implantation window

Mateusz Mikołajczyk, Jana Skrzypczak, Przemysław Wirstlein
DOI: 10.5603/FHC.2011.0084
·
Folia Histochem Cytobiol 2011;49(4):615-621.

open access

Vol 49, No 4 (2011)
ORIGINAL PAPERS
Submitted: 2012-01-16
Published online: 2012-01-16

Abstract

Implantation depends on two factors — embryo and endometrium. The period of maximal endometrial receptivity is a poorly understood phenomenon. We decided to look at three possible markers of implantation: pinopodes, leukemia inhibitory factor, and matrix metalloproteinase 2 and their correlations. We included in the study 23 idiopathic infertility patients and 21 patients with recurrent spontaneous abortions of unknown etiology. Twenty one fertile patients were also recruited. A biopsy was used for endometrial dating according to the Noyes and Hertig criteria, and assessed for the presence of pinopodes via a scanning electron microscope. Endometria were examined in Real Time-Polymerase Chain Reaction cycles for the mRNA expression of leukemia inhibitory factor (LIF) and matrix metalloproteinase 2 (MMP2). No difference was found in the stage of pinopodes development, nor in the coverage of endometrial surface between the studied groups. The expression level for LIF mRNA was lower in control patients compared to idiopathic infertility and recurrent miscarriage patients. No difference was detected in the expression of MMP2 between all studied groups. No correlation was found between pinopodes development stage and LIF and MMP2 expressions in endometrium. Of the studied factors, LIF and pinopodes show the most promise as potential markers of endometrial receptivity. However, the results achieved suggest that these markers are independent of each other. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 4, pp. 615–621)

Abstract

Implantation depends on two factors — embryo and endometrium. The period of maximal endometrial receptivity is a poorly understood phenomenon. We decided to look at three possible markers of implantation: pinopodes, leukemia inhibitory factor, and matrix metalloproteinase 2 and their correlations. We included in the study 23 idiopathic infertility patients and 21 patients with recurrent spontaneous abortions of unknown etiology. Twenty one fertile patients were also recruited. A biopsy was used for endometrial dating according to the Noyes and Hertig criteria, and assessed for the presence of pinopodes via a scanning electron microscope. Endometria were examined in Real Time-Polymerase Chain Reaction cycles for the mRNA expression of leukemia inhibitory factor (LIF) and matrix metalloproteinase 2 (MMP2). No difference was found in the stage of pinopodes development, nor in the coverage of endometrial surface between the studied groups. The expression level for LIF mRNA was lower in control patients compared to idiopathic infertility and recurrent miscarriage patients. No difference was detected in the expression of MMP2 between all studied groups. No correlation was found between pinopodes development stage and LIF and MMP2 expressions in endometrium. Of the studied factors, LIF and pinopodes show the most promise as potential markers of endometrial receptivity. However, the results achieved suggest that these markers are independent of each other. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 4, pp. 615–621)

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Keywords

infertility; recurrent miscarriage; pinopodes; endometrium; LIF; MMP; idiopathic infertility

About this article
Title

No correlation between pinopode formation and LIF and MMP2 expression in endometrium during implantation window

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 49, No 4 (2011)

Article type

Original paper

Pages

615-621

Published online

2012-01-16

Page views

2085

Article views/downloads

2277

DOI

10.5603/FHC.2011.0084

Bibliographic record

Folia Histochem Cytobiol 2011;49(4):615-621.

Keywords

infertility
recurrent miscarriage
pinopodes
endometrium
LIF
MMP
idiopathic infertility

Authors

Mateusz Mikołajczyk
Jana Skrzypczak
Przemysław Wirstlein

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