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open access

Vol 73, No 5 (2022)
Original paper
Submitted: 2022-02-28
Accepted: 2022-05-09
Published online: 2022-07-27
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Low miR-222 expression in human visceral adipose tissue is associated with insulin resistance and PTEN and p53 mRNA levels

Paulina Estrella-Ibarra12, Andrés Cruz-Hernández3, Ana Karen Torres-Arreola12, Francisco Campos-Maldonado12, Adriana Jheny Rodríguez-Mendez2, Olga Patricia García-Obregón4, Juan Carlos Solís-Sáinz2, María del Carmen Aburto-Fernández5, Ricardo M. Lerma-Alvarado5, Pablo García-Solís2
DOI: 10.5603/EP.a2022.0057
·
Pubmed: 35971931
·
Endokrynol Pol 2022;73(5):846-855.
Affiliations
  1. Biological Sciences PhD Program, School of Natural Sciences, Autonomous University of Queretaro, Queretaro, Mexico
  2. Center of Advanced Biomedical Research, School of Medicine, Autonomous University of Queretaro, Queretaro, Mexico
  3. Agronomy School, De La Salle Bajio University, Guanajuato, Mexico
  4. Department of Human Nutrition, School of Natural Sciences, Autonomous University of Queretaro, Queretaro, Mexico
  5. Department of General Surgery, General Hospital of Queretaro, Queretaro, Mexico

open access

Vol 73, No 5 (2022)
Original Paper
Submitted: 2022-02-28
Accepted: 2022-05-09
Published online: 2022-07-27

Abstract

Introduction: The aim of this is study was to analyse the expression of miR-193b, miR-378, miR-Let7-d, and miR-222 in human visceral adipose tissue (VAT), as well as their association with obesity, insulin resistance (IR), and their role in the regulation of genes controlling adipose tissue homeostasis, including adipocytokines, the phosphatase and tension homologue (PTEN), and tumour protein 53 (p53).

Material and methods: VAT was obtained from normal-weight (NW), overweight, and obese (OW/OB) subjects with and without IR. Stem-loop RT-qPCR was used to evaluate miRNA expression levels. miRTarBase 4.0, miRWalk, and DIANA-TarBase v8 were used for prediction of validated target gene of the miRNA analysed. A qPCR was used to evaluate PTEN, p53, leptin (LEP), and adiponectin (ADIPOQ) mRNA.

Results: miR-222 was lower in IR subjects, and miR-222 and miR-378 negatively correlated with HOMA-IR. PTEN and p53 are miR-222 direct targets according to databases. mRNA expression of PTEN and p53 was lower in OW/OB subjects with and without IR, compared to NW group and its levels positively associated with miR-222. Additionally, p53 and PTEN are positively associated with serum leptin levels. On the other hand, miR-193b and miR-378 negatively correlated with serum leptin but not with mRNA levels. Moreover, miR-Let-7d negatively correlated with serum adiponectin but not with adiponectin mRNA levels.

Conclusions: Lower miR-222 levels are associated with IR, and PTEN and p53 expression; the implication of these genes in adipose tissue homeostasis needs more research.

Abstract

Introduction: The aim of this is study was to analyse the expression of miR-193b, miR-378, miR-Let7-d, and miR-222 in human visceral adipose tissue (VAT), as well as their association with obesity, insulin resistance (IR), and their role in the regulation of genes controlling adipose tissue homeostasis, including adipocytokines, the phosphatase and tension homologue (PTEN), and tumour protein 53 (p53).

Material and methods: VAT was obtained from normal-weight (NW), overweight, and obese (OW/OB) subjects with and without IR. Stem-loop RT-qPCR was used to evaluate miRNA expression levels. miRTarBase 4.0, miRWalk, and DIANA-TarBase v8 were used for prediction of validated target gene of the miRNA analysed. A qPCR was used to evaluate PTEN, p53, leptin (LEP), and adiponectin (ADIPOQ) mRNA.

Results: miR-222 was lower in IR subjects, and miR-222 and miR-378 negatively correlated with HOMA-IR. PTEN and p53 are miR-222 direct targets according to databases. mRNA expression of PTEN and p53 was lower in OW/OB subjects with and without IR, compared to NW group and its levels positively associated with miR-222. Additionally, p53 and PTEN are positively associated with serum leptin levels. On the other hand, miR-193b and miR-378 negatively correlated with serum leptin but not with mRNA levels. Moreover, miR-Let-7d negatively correlated with serum adiponectin but not with adiponectin mRNA levels.

Conclusions: Lower miR-222 levels are associated with IR, and PTEN and p53 expression; the implication of these genes in adipose tissue homeostasis needs more research.

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Keywords

microRNA (miRNA); visceral adipose tissue; obesity; insulin resistance; adipocytokines

About this article
Title

Low miR-222 expression in human visceral adipose tissue is associated with insulin resistance and PTEN and p53 mRNA levels

Journal

Endokrynologia Polska

Issue

Vol 73, No 5 (2022)

Article type

Original paper

Pages

846-855

Published online

2022-07-27

Page views

4363

Article views/downloads

425

DOI

10.5603/EP.a2022.0057

Pubmed

35971931

Bibliographic record

Endokrynol Pol 2022;73(5):846-855.

Keywords

microRNA (miRNA)
visceral adipose tissue
obesity
insulin resistance
adipocytokines

Authors

Paulina Estrella-Ibarra
Andrés Cruz-Hernández
Ana Karen Torres-Arreola
Francisco Campos-Maldonado
Adriana Jheny Rodríguez-Mendez
Olga Patricia García-Obregón
Juan Carlos Solís-Sáinz
María del Carmen Aburto-Fernández
Ricardo M. Lerma-Alvarado
Pablo García-Solís

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