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Biological functions of nuclear-localized insulin receptor (IR) on A549 lung cancer cells
Affiliations- Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China
- Department of Respiratory, Hei long Jiang Province Hospital, Harbin, Hei long Jiang, China
- Hei Long Jiang College of Business and Technology, Hei Long Jiang, China
- Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China
open access
Abstract
Introduction: Under normal physiological conditions, insulin exhibits a series of important biological functions and roles. Recent studies have shown that insulin is also closely related to the occurrence and development of lung cancer. However, until now, the cellular properties of insulin/insulin receptor (IR) on lung cancer have not been fully revealed.
Material and methods: Indirect immunofluorescence, western blot, and other techniques have been used to identify the biological activity of insulin on lung cancer cell lines.
Results: The biological activities of insulin are closely related to its cell behaviour. Therefore, we used lung cancer cell lines as a model to explore the cellular behaviour and properties of insulin/IR in the current study, and the results showed that the IR could internalize into lung cancer cells, and it can also transport into the cell nuclei under insulin treatment. Further study showed that nuclear-localized IR could promote the proliferation of lung cancer cells.
Conclusion: Taken together, this study shows that IR’s nuclear localization is closely related to cell proliferation. This work lays the foundation for further research on the relationship between insulin and the occurrence and development of lung cancer.
Abstract
Introduction: Under normal physiological conditions, insulin exhibits a series of important biological functions and roles. Recent studies have shown that insulin is also closely related to the occurrence and development of lung cancer. However, until now, the cellular properties of insulin/insulin receptor (IR) on lung cancer have not been fully revealed.
Material and methods: Indirect immunofluorescence, western blot, and other techniques have been used to identify the biological activity of insulin on lung cancer cell lines.
Results: The biological activities of insulin are closely related to its cell behaviour. Therefore, we used lung cancer cell lines as a model to explore the cellular behaviour and properties of insulin/IR in the current study, and the results showed that the IR could internalize into lung cancer cells, and it can also transport into the cell nuclei under insulin treatment. Further study showed that nuclear-localized IR could promote the proliferation of lung cancer cells.
Conclusion: Taken together, this study shows that IR’s nuclear localization is closely related to cell proliferation. This work lays the foundation for further research on the relationship between insulin and the occurrence and development of lung cancer.
Keywords
insulin; insulin receptor; lung cancer
About this articleTitle
Biological functions of nuclear-localized insulin receptor (IR) on A549 lung cancer cells
Journal
Issue
Article type
Original paper
Pages
121-130
Published online
2021-11-04
Page views
5821
Article views/downloads
386
DOI
Pubmed
Bibliographic record
Endokrynol Pol 2022;73(1):121-130.
Keywords
insulin
insulin receptor
lung cancer
Authors
Ren Qiu
Tian Miao
Lin Lin
Zhang Wei
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