open access

Vol 70, No 4 (2019)
Original papers
Published online: 2019-04-02
Submitted: 2019-01-29
Accepted: 2019-03-01
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The level of IL-35 in the circulation of patients with Graves’ disease

Meng Shuai, Yan Ni, Xu Jian, Shuangtao He, Jin-an Zhang
DOI: 10.5603/EP.a2019.0018
·
Pubmed: 30938833
·
Endokrynologia Polska 2019;70(4):318-322.

open access

Vol 70, No 4 (2019)
Original papers
Published online: 2019-04-02
Submitted: 2019-01-29
Accepted: 2019-03-01

Abstract

Introduction: The aim of this study was to explore the potential role of IL-35 in Graves’ disease (GD).

Material and methods: A total of 142 GD patients including 80 newly onset patients, 52 refractory patients and 10 remission patients and 70 normal controls (NCs) were recruited. The messenger RNA (mRNA) expressions of P35 and Epstein-Barr-virus-induced gene 3 (Ebi3) were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Serum level of IL-35 was measured by enzyme-linked immunosorbent assay (ELISA).

Results: The expression of IL-35mRNA in new onset GD and refractory GD were both significantly higher than NC. Comparison between remission GD and NC showed no significant difference (p > 0.05). A significant increase of Ebi3mRNA expression was observed in new onset GD compared with remission GD (p = 0.030). The new onset GD showed a tendency for increased expression of serum IL-35 but without significant difference. No correlation between IL-35 expression and clinic parameters was found.

Conclusions: Our preliminary observations indicate that IL-35 and CD4+P35+Ebi3+T cells may be involved in the pathogenesis of GD.

Abstract

Introduction: The aim of this study was to explore the potential role of IL-35 in Graves’ disease (GD).

Material and methods: A total of 142 GD patients including 80 newly onset patients, 52 refractory patients and 10 remission patients and 70 normal controls (NCs) were recruited. The messenger RNA (mRNA) expressions of P35 and Epstein-Barr-virus-induced gene 3 (Ebi3) were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Serum level of IL-35 was measured by enzyme-linked immunosorbent assay (ELISA).

Results: The expression of IL-35mRNA in new onset GD and refractory GD were both significantly higher than NC. Comparison between remission GD and NC showed no significant difference (p > 0.05). A significant increase of Ebi3mRNA expression was observed in new onset GD compared with remission GD (p = 0.030). The new onset GD showed a tendency for increased expression of serum IL-35 but without significant difference. No correlation between IL-35 expression and clinic parameters was found.

Conclusions: Our preliminary observations indicate that IL-35 and CD4+P35+Ebi3+T cells may be involved in the pathogenesis of GD.

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Keywords

interleukin 35; CD4+P35+Ebi3+T cell; Graves’ disease; immunology

About this article
Title

The level of IL-35 in the circulation of patients with Graves’ disease

Journal

Endokrynologia Polska

Issue

Vol 70, No 4 (2019)

Pages

318-322

Published online

2019-04-02

DOI

10.5603/EP.a2019.0018

Pubmed

30938833

Bibliographic record

Endokrynologia Polska 2019;70(4):318-322.

Keywords

interleukin 35
CD4+P35+Ebi3+T cell
Graves’ disease
immunology

Authors

Meng Shuai
Yan Ni
Xu Jian
Shuangtao He
Jin-an Zhang

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