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open access

Vol 71, No 3 (2020)
Original paper
Submitted: 2019-12-27
Accepted: 2020-03-27
Published online: 2020-05-12
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Identification of related long non-coding RNAs and mRNAs in subclinical hypothyroidism complicated with type 2 diabetes by transcriptome analysis — a preliminary study

Qiang Jiang123, Lizhi Sun3, Yong Lu1, Shuyi Han3, Lulu Hou1, Kai Lou1, Jianting Li1, Lulu Wang1, Shuguang Pang1
DOI: 10.5603/EP.a2020.0025
·
Pubmed: 36624669
·
Endokrynol Pol 2020;71(3):213-226.
Affiliations
  1. Department of Endocrinology, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China
  2. Department of Endocrinology, Jinan Central Hospital Affliated to Shandong First Medical University, Jinan, China
  3. Central Laboratory, Jinan central Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China

open access

Vol 71, No 3 (2020)
Original Paper
Submitted: 2019-12-27
Accepted: 2020-03-27
Published online: 2020-05-12

Abstract

Introduction: The pathology mechanism of subclinical hypothyroidism and subclinical hypothyroidism complicated with type 2 diabetes remained uncertain. We aimed to find potential related long non-coding RNAs (lncRNAs) and mRNAs in the above diseases.

Material and methods: Transcriptome sequencing was performed in three patients with subclinical hypothyroidism (S), three patients with subclinical hypothyroidism complicated with type 2 diabetes (SD), and three healthy controls (N). Differentially expressed mRNAs (DEmRNAs) and differentially expressed lncRNAs (DElncRNAs) were screened in S vs. N, SD vs. N, and SD vs. S group, and the nearby and co-expressed DEmRNAs of DElncRNAs were screened in S vs. N and SD vs. N. Moreover, functional analysis of DEmRNAs was then performed by Metascape.

Results: In total, 465, 1058, and 943 DEmRNAs were obtained in S vs. N, SD vs. N, SD vs. S, respectively, and 191 overlapping genes were obtained in S vs. N and SD vs. N group. Among which, LAIR2, PNMA6A, and SFRP2 were deduced to be involved in subclinical hypothyroidism, and GPR162, APOL4, and ANK1 were deduced to be associated with subclinical hypothyroidism complicated with type 2 diabetes. A total of 50, 100, and 88 DElncRNAs were obtained in S vs. N, SD vs. N and SD vs. S, respectively. Combining with the interaction network of DElncRNA-DEmRNA, PAX8-AS1, co-expressed with KIR3DL1, was identified to function in subclinical hypothyroidism, and JHDM1D-AS1, co-expressed with ANK1, was deduced to play a role in subclinical hypothyroidism complicated with type 2 diabetes.

Conclusions: Dysfunctional lncRNAs and mRNAs may be involved in the development of subclinical hypothyroidism and subclinical hypothyroidism complicated with type 2 diabetes. 

Abstract

Introduction: The pathology mechanism of subclinical hypothyroidism and subclinical hypothyroidism complicated with type 2 diabetes remained uncertain. We aimed to find potential related long non-coding RNAs (lncRNAs) and mRNAs in the above diseases.

Material and methods: Transcriptome sequencing was performed in three patients with subclinical hypothyroidism (S), three patients with subclinical hypothyroidism complicated with type 2 diabetes (SD), and three healthy controls (N). Differentially expressed mRNAs (DEmRNAs) and differentially expressed lncRNAs (DElncRNAs) were screened in S vs. N, SD vs. N, and SD vs. S group, and the nearby and co-expressed DEmRNAs of DElncRNAs were screened in S vs. N and SD vs. N. Moreover, functional analysis of DEmRNAs was then performed by Metascape.

Results: In total, 465, 1058, and 943 DEmRNAs were obtained in S vs. N, SD vs. N, SD vs. S, respectively, and 191 overlapping genes were obtained in S vs. N and SD vs. N group. Among which, LAIR2, PNMA6A, and SFRP2 were deduced to be involved in subclinical hypothyroidism, and GPR162, APOL4, and ANK1 were deduced to be associated with subclinical hypothyroidism complicated with type 2 diabetes. A total of 50, 100, and 88 DElncRNAs were obtained in S vs. N, SD vs. N and SD vs. S, respectively. Combining with the interaction network of DElncRNA-DEmRNA, PAX8-AS1, co-expressed with KIR3DL1, was identified to function in subclinical hypothyroidism, and JHDM1D-AS1, co-expressed with ANK1, was deduced to play a role in subclinical hypothyroidism complicated with type 2 diabetes.

Conclusions: Dysfunctional lncRNAs and mRNAs may be involved in the development of subclinical hypothyroidism and subclinical hypothyroidism complicated with type 2 diabetes. 

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Keywords

 subclinical hypothyroidism; subclinical hypothyroidism complicated with type 2 diabetes; lncRNAs; mRNAs

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Title

Identification of related long non-coding RNAs and mRNAs in subclinical hypothyroidism complicated with type 2 diabetes by transcriptome analysis — a preliminary study

Journal

Endokrynologia Polska

Issue

Vol 71, No 3 (2020)

Article type

Original paper

Pages

213-226

Published online

2020-05-12

Page views

829

Article views/downloads

804

DOI

10.5603/EP.a2020.0025

Pubmed

36624669

Bibliographic record

Endokrynol Pol 2020;71(3):213-226.

Keywords

 subclinical hypothyroidism
subclinical hypothyroidism complicated with type 2 diabetes
lncRNAs
mRNAs

Authors

Qiang Jiang
Lizhi Sun
Yong Lu
Shuyi Han
Lulu Hou
Kai Lou
Jianting Li
Lulu Wang
Shuguang Pang

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