Vol 1, No 2 (2012)
Original articles (submitted)
Published online: 2012-06-08
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Blood serum of patients with diabetic kidney disease stimulates thrombospondin 1 gene expression in peripheral blood mononuclear cells

Dariusz Kazimierz Moczulski
Diabetologia Kliniczna 2012;1(2).
Vol 1, No 2 (2012)
Original articles (submitted)
Published online: 2012-06-08

Abstract

Background: The thrombospondin 1 and cyclooxygenase 1 genes were over-expressed and the matrix metalloproteinase 9 and cyclooxygenase 2 genes have lower expression in peripheral blood mononuclear cells (PBMC) of type 1 diabetic patients with diabetic kidney disease (DKD). The aim of the study was to examine if the expression of those genes could be stimulate by incubating PBMC of healthy volunteers in blood serum of patients with DKD.

Material and Methods: Blood serum samples from five type 2 diabetic patients with DKD and five type 2 diabetic patients without DKD were collected. PBMC of four healthy volunteers were incubated in blood serum of 5 type 2 diabetic patients with DKD and 5 without DKD. The expression of the thrombospondin 1, cyclooxygenase 1, matrix metalloproteinase 9 and cyclooxygenase 2 genes was examined using a method based on the real-time PCR. The expression was compared between cells incubated in blood serum of type 2 diabetic patients with and without DKD.

Results: We observed a higher expression of the thrombospondin 1 gene in cells incubated in blood serum of patients with DKD. The expression of other genes did not differ between cells incubated in blood serum of patients with and without DKD.

Conclusions: Blood serum of type 2 diabetic patients with DKD induces expression of thrombospondin 1 gene in PBMC. Blood serum of type 2 diabetes patients with DKD may contain an intrinsic factor which may be involved in vascular complications accelerated by DKD.

 

Abstract

Background: The thrombospondin 1 and cyclooxygenase 1 genes were over-expressed and the matrix metalloproteinase 9 and cyclooxygenase 2 genes have lower expression in peripheral blood mononuclear cells (PBMC) of type 1 diabetic patients with diabetic kidney disease (DKD). The aim of the study was to examine if the expression of those genes could be stimulate by incubating PBMC of healthy volunteers in blood serum of patients with DKD.

Material and Methods: Blood serum samples from five type 2 diabetic patients with DKD and five type 2 diabetic patients without DKD were collected. PBMC of four healthy volunteers were incubated in blood serum of 5 type 2 diabetic patients with DKD and 5 without DKD. The expression of the thrombospondin 1, cyclooxygenase 1, matrix metalloproteinase 9 and cyclooxygenase 2 genes was examined using a method based on the real-time PCR. The expression was compared between cells incubated in blood serum of type 2 diabetic patients with and without DKD.

Results: We observed a higher expression of the thrombospondin 1 gene in cells incubated in blood serum of patients with DKD. The expression of other genes did not differ between cells incubated in blood serum of patients with and without DKD.

Conclusions: Blood serum of type 2 diabetic patients with DKD induces expression of thrombospondin 1 gene in PBMC. Blood serum of type 2 diabetes patients with DKD may contain an intrinsic factor which may be involved in vascular complications accelerated by DKD.

 

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About this article
Title

Blood serum of patients with diabetic kidney disease stimulates thrombospondin 1 gene expression in peripheral blood mononuclear cells

Journal

Clinical Diabetology

Issue

Vol 1, No 2 (2012)

Published online

2012-06-08

Bibliographic record

Diabetologia Kliniczna 2012;1(2).

Authors

Dariusz Kazimierz Moczulski

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