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Research paper
Published online: 2019-09-04
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Infiltration of CD68+ cells correlates positively with matrix metalloproteinase 2 expression in the arteries used as aortocoronary bypass grafts. Possible clinical implications

Bartłomiej Perek, Katarzyna Kowalska, Bartosz Kempisty, Mariusz Nawrocki, Michał Nowicki, Mateusz Puślecki, Danuta Ostalska-Nowicka, Łukasz Szarpak, Navid Ahmadi, Agnieszka Malińska
DOI: 10.5603/CJ.a2019.0087
·
Pubmed: 31489609

open access

Ahead of print
Original articles
Published online: 2019-09-04

Abstract

Background: Late failure of arterial aortocoronary conduits may result from abnormal activity of cells found in the vessel wall, including macrophages. The purpose of this study was to assess if there are any associations between the number of macrophages and overexpression of matrix metalloproteinases (MMPs) in the wall of artery grafts, as well as their clinical significance.

Methods: This study involved 128 consecutive patients with a mean age of 64.9 ± 9.7 years who underwent elective surgery for coronary artery disease (CAD). The surplus segments of internal thoracic artery (ITA) and radial arteries (RA) were taken for immunohistochemical analysis of macrophage numbers and MMPs expression. The participants who reached the clinical primary end-point (cardiac-related death, acute coronary syndrome or progression of CAD) had a follow-up angiography.

Results: The mean numbers of macrophages were higher on RA (70 [24; 112]) than ITA cross-sections (44 [24; 59]; p < 0.001). Median expression of both MMP2 and MMP9 were stronger in the ITA than RA cross-sections (p < 0.001). A significant positive correlation of MMP2 expression and a number of macrophages infiltrating the tunica media of arterial segments were noted on both ITA and RA cross-sections. In addition, the arterial segments of the 6 patients who reached clinical end-point had higher numbers of macrophages and stronger MMP2 expression when compared to the rest of the participants.

Conclusions: Macrophage infiltration of arterial wall grafts prior to harvesting may be associated with higher risk of late occlusion and MMP 2 might be facilitating this process.

Abstract

Background: Late failure of arterial aortocoronary conduits may result from abnormal activity of cells found in the vessel wall, including macrophages. The purpose of this study was to assess if there are any associations between the number of macrophages and overexpression of matrix metalloproteinases (MMPs) in the wall of artery grafts, as well as their clinical significance.

Methods: This study involved 128 consecutive patients with a mean age of 64.9 ± 9.7 years who underwent elective surgery for coronary artery disease (CAD). The surplus segments of internal thoracic artery (ITA) and radial arteries (RA) were taken for immunohistochemical analysis of macrophage numbers and MMPs expression. The participants who reached the clinical primary end-point (cardiac-related death, acute coronary syndrome or progression of CAD) had a follow-up angiography.

Results: The mean numbers of macrophages were higher on RA (70 [24; 112]) than ITA cross-sections (44 [24; 59]; p < 0.001). Median expression of both MMP2 and MMP9 were stronger in the ITA than RA cross-sections (p < 0.001). A significant positive correlation of MMP2 expression and a number of macrophages infiltrating the tunica media of arterial segments were noted on both ITA and RA cross-sections. In addition, the arterial segments of the 6 patients who reached clinical end-point had higher numbers of macrophages and stronger MMP2 expression when compared to the rest of the participants.

Conclusions: Macrophage infiltration of arterial wall grafts prior to harvesting may be associated with higher risk of late occlusion and MMP 2 might be facilitating this process.

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Keywords

coronary artery bypass grafting, arterial aortocoronary grafts, macrophage, matrix metalloproteinase, outcomes

About this article
Title

Infiltration of CD68+ cells correlates positively with matrix metalloproteinase 2 expression in the arteries used as aortocoronary bypass grafts. Possible clinical implications

Journal

Cardiology Journal

Issue

Ahead of print

Article type

Research paper

Published online

2019-09-04

DOI

10.5603/CJ.a2019.0087

Pubmed

31489609

Keywords

coronary artery bypass grafting
arterial aortocoronary grafts
macrophage
matrix metalloproteinase
outcomes

Authors

Bartłomiej Perek
Katarzyna Kowalska
Bartosz Kempisty
Mariusz Nawrocki
Michał Nowicki
Mateusz Puślecki
Danuta Ostalska-Nowicka
Łukasz Szarpak
Navid Ahmadi
Agnieszka Malińska

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