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open access

Vol 27, No 3 (2020)
Original articles — Basic science and experimental cardiology
Submitted: 2018-05-24
Accepted: 2018-09-28
Published online: 2019-04-11
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MiR-1/133 attenuates cardiomyocyte apoptosis and electrical remodeling in mice with viral myocarditis

Wei Li1, Mengmeng Liu2, Cuifen Zhao2, Cai Chen1, Qingyu Kong2, Zhifeng Cai2, Dong Li3
DOI: 10.5603/CJ.a2019.0036
·
Pubmed: 30994182
·
Cardiol J 2020;27(3):285-294.
Affiliations
  1. Biomedical Engineering Institute, School of Control Science and Engineering, Shandong University, Jinan, China
  2. Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, China
  3. Research Room of Hypothermia Medicine, Qilu Hospital of Shandong University, Jinan, China

open access

Vol 27, No 3 (2020)
Original articles — Basic science and experimental cardiology
Submitted: 2018-05-24
Accepted: 2018-09-28
Published online: 2019-04-11

Abstract

Background: The role of miR-1 and miR-133 in regulating the expression of potassium and calcium ion channels, and mediating cardiomyocyte apoptosis in mice with viral myocarditis (VMC) is investigated herein.

Methods: Male Balb/c mice were randomly divided into groups: control group, VMC group, VMC + miR-1/133 mimics group, or VMC + miR-1/133 negative control (NC) group. VMC was induced with coxsackievirus B3 (CVB3). MiR-1/133 mimics ameliorated cardiac dysfunction in VMC mice and was compared to the VMC+NC group.

Results: Hematoxylin and eosin staining showed a well-arranged myocardium without inflammatory cell infiltration in the myocardial matrix of the control group. However, in the VMC and VMC+NC groups, the myocardium was disorganized and swollen with necrosis, and the myocardial matrix was infiltrated with inflammatory cells. These changes were alleviated by miR-1/133 mimics. TUNEL staining revealed decreased cardiomyocyte apoptosis in the VMC + miR-1/133 mimics group compared with the VMC group. In addition, miR-1/133 mimics up-regulated the expression of miR-1 and miR-133, the potassium channel genes Kcnd2 and Kcnj2, as well as Bcl-2, and down-regulated the expression of the potassium channel suppressor gene Irx5, L-type calcium channel subunit gene a1c (Cacna1c), Bax, and caspase-9 in the myocardium of VMC mice. MiR-1/133 also up-regulated the protein levels of Kv4.2 and Kir2.1, and down-regulated the expression of CaV1.2 in the myocardium of VMC mice.

Conclusions: MiR-1 and miR-133 decreased cardiomyocyte apoptosis by mediating the expression of apoptosis-related genes in the hearts of VMC mice.

Abstract

Background: The role of miR-1 and miR-133 in regulating the expression of potassium and calcium ion channels, and mediating cardiomyocyte apoptosis in mice with viral myocarditis (VMC) is investigated herein.

Methods: Male Balb/c mice were randomly divided into groups: control group, VMC group, VMC + miR-1/133 mimics group, or VMC + miR-1/133 negative control (NC) group. VMC was induced with coxsackievirus B3 (CVB3). MiR-1/133 mimics ameliorated cardiac dysfunction in VMC mice and was compared to the VMC+NC group.

Results: Hematoxylin and eosin staining showed a well-arranged myocardium without inflammatory cell infiltration in the myocardial matrix of the control group. However, in the VMC and VMC+NC groups, the myocardium was disorganized and swollen with necrosis, and the myocardial matrix was infiltrated with inflammatory cells. These changes were alleviated by miR-1/133 mimics. TUNEL staining revealed decreased cardiomyocyte apoptosis in the VMC + miR-1/133 mimics group compared with the VMC group. In addition, miR-1/133 mimics up-regulated the expression of miR-1 and miR-133, the potassium channel genes Kcnd2 and Kcnj2, as well as Bcl-2, and down-regulated the expression of the potassium channel suppressor gene Irx5, L-type calcium channel subunit gene a1c (Cacna1c), Bax, and caspase-9 in the myocardium of VMC mice. MiR-1/133 also up-regulated the protein levels of Kv4.2 and Kir2.1, and down-regulated the expression of CaV1.2 in the myocardium of VMC mice.

Conclusions: MiR-1 and miR-133 decreased cardiomyocyte apoptosis by mediating the expression of apoptosis-related genes in the hearts of VMC mice.

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Keywords

miR-1, miR-133, viral myocarditis, ion channels, cell apoptosis infarction, coronary, psoriasis, anaphylaxis

About this article
Title

MiR-1/133 attenuates cardiomyocyte apoptosis and electrical remodeling in mice with viral myocarditis

Journal

Cardiology Journal

Issue

Vol 27, No 3 (2020)

Pages

285-294

Published online

2019-04-11

Page views

2494

Article views/downloads

1250

DOI

10.5603/CJ.a2019.0036

Pubmed

30994182

Bibliographic record

Cardiol J 2020;27(3):285-294.

Keywords

miR-1
miR-133
viral myocarditis
ion channels
cell apoptosis infarction
coronary
psoriasis
anaphylaxis

Authors

Wei Li
Mengmeng Liu
Cuifen Zhao
Cai Chen
Qingyu Kong
Zhifeng Cai
Dong Li

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