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Vol 27, No 3 (2020)
Original articles — Basic science and experimental cardiology
Submitted: 2018-01-19
Accepted: 2018-09-04
Published online: 2018-09-13
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Characteristics of circulating endothelial cells obtained from non-ST-segment elevation myocardial infarction patients with additional diastolic dysfunction of left ventricle observed in echocardiography

Pawel Burchardt123, Maura Farinacci4, Magdalena Mayer5, Klaus Luecke6, Thomas Krahn7, Jaroslaw Manczak2, Marek Slomczynski2, Jaroslaw Hiczkiewicz3, Janusz Rzezniczak2
DOI: 10.5603/CJ.a2018.0110
·
Pubmed: 30234891
·
Cardiol J 2020;27(3):295-302.
Affiliations
  1. Biology of Lipid Disorders Department, Chair of Biology and Environmental Sciences, Poznan University of Medical Sciences, Poznan, Poland
  2. Department of Cardiology, J. Strus Hospital, Szwajcarska 3, 61-285 Poznan, Poland
  3. Department of Cardiology, Hospital of Nowa Sol, Poland
  4. Institute for Medical Immunology, Berlin-Brandenburg Center für Regenerative Therapies, Core Unit Biomarker, Charité University Medicine, Berlin, Germany
  5. Zablab sp. z o.o, Poznan, Poland
  6. HaimaChek Inc, Santa Monica, Santa Monica, CA,, United States
  7. Bayer AG, Biomarker Research, Berlin, Germany

open access

Vol 27, No 3 (2020)
Original articles — Basic science and experimental cardiology
Submitted: 2018-01-19
Accepted: 2018-09-04
Published online: 2018-09-13

Abstract

Background: Circulating endothelial cells (CEC) may be used to find new strategies for the early di­agnosis of cardiovascular diseases. The major objective of the project is to broaden knowledge of CEC biology by determining their phenotypic characteristics. The additional aim is to clarify whether on the basis of these information it is possible to identify the origin of CEC release (from various cardiovascular compartments).

Methods: Circulating endothelial cells were collected from arterial blood prior to angiography, as well as from arterial and venous blood obtained after angiography/coronary angioplasty, from 18 patients with non-ST-segment elevation myocardial infarction (NSTEMI). CECs were quantified by flow cytometry and defined as Syto16 (dye)+, CD45dim/neg, CD31+ and CD146+. The additional CD36+ was establish as a marker of endothelial cells released from small vessels of the microcirculation.

Results: The total number of CECs increased significantly after the percutaneous transluminal coronary angioplasty (PTCA) in the arterial system. Number of CECs isolated at similar time points (after invasive procedure) did not differ significantly between arteries and veins, but the number of CD36+ CECs after coronary angioplasty was significantly higher in the venous system, than in the arterial system.

Conclusions: The number of CD36+ in artery samples obtained after coronary angioplasty (PTCA) had tendency to be decreased (in comparison to the sample obtained before angiography). It was major difference between those who had PTCA performed vs. those who had not.

Abstract

Background: Circulating endothelial cells (CEC) may be used to find new strategies for the early di­agnosis of cardiovascular diseases. The major objective of the project is to broaden knowledge of CEC biology by determining their phenotypic characteristics. The additional aim is to clarify whether on the basis of these information it is possible to identify the origin of CEC release (from various cardiovascular compartments).

Methods: Circulating endothelial cells were collected from arterial blood prior to angiography, as well as from arterial and venous blood obtained after angiography/coronary angioplasty, from 18 patients with non-ST-segment elevation myocardial infarction (NSTEMI). CECs were quantified by flow cytometry and defined as Syto16 (dye)+, CD45dim/neg, CD31+ and CD146+. The additional CD36+ was establish as a marker of endothelial cells released from small vessels of the microcirculation.

Results: The total number of CECs increased significantly after the percutaneous transluminal coronary angioplasty (PTCA) in the arterial system. Number of CECs isolated at similar time points (after invasive procedure) did not differ significantly between arteries and veins, but the number of CD36+ CECs after coronary angioplasty was significantly higher in the venous system, than in the arterial system.

Conclusions: The number of CD36+ in artery samples obtained after coronary angioplasty (PTCA) had tendency to be decreased (in comparison to the sample obtained before angiography). It was major difference between those who had PTCA performed vs. those who had not.

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Keywords

circulating endothelial cells, NSTEMI, diastolic dysfunction

About this article
Title

Characteristics of circulating endothelial cells obtained from non-ST-segment elevation myocardial infarction patients with additional diastolic dysfunction of left ventricle observed in echocardiography

Journal

Cardiology Journal

Issue

Vol 27, No 3 (2020)

Pages

295-302

Published online

2018-09-13

Page views

1356

Article views/downloads

624

DOI

10.5603/CJ.a2018.0110

Pubmed

30234891

Bibliographic record

Cardiol J 2020;27(3):295-302.

Keywords

circulating endothelial cells
NSTEMI
diastolic dysfunction

Authors

Pawel Burchardt
Maura Farinacci
Magdalena Mayer
Klaus Luecke
Thomas Krahn
Jaroslaw Manczak
Marek Slomczynski
Jaroslaw Hiczkiewicz
Janusz Rzezniczak

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