open access

Vol 25, No 6 (2018)
Original articles — Basic science and experimental cardiology
Published online: 2017-08-24
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Right ventricular morphology and function is not related with microRNAs and fibrosis markers in dilated cardiomyopathy

Paweł Rubiś, Justyna Totoń-Żurańska, Sylwia Wiśniowska-Śmiałek, Maria Kołton-Wróż, Paweł Wołkow, Ewa Wypasek, Lucyna Rudnicka-Sosin, Agnieszka Pawlak, Kozanecki Kozanecki, Lidia Tomkiewicz-Pająk, Piotr Podolec
DOI: 10.5603/CJ.a2017.0099
·
Pubmed: 28840590
·
Cardiol J 2018;25(6):722-731.

open access

Vol 25, No 6 (2018)
Original articles — Basic science and experimental cardiology
Published online: 2017-08-24

Abstract

Background: The relationship between right ventricle (RV), extracellular matrix (ECM) fibrosis and fibrosis-linked, circulating microRNAs in dilated cardiomyopathy (DCM) is unknown.

Aim: The aim of the study was to uncover the associations between serum markers of ECM metabolism and circulating microRNAs with RV morphological and functional parameters.

Methods: The study population consisted of 70 consecutive DCM patients (ejection fraction 24.4 ± ± 7.4%). Based on detailed echocardiographic assessment — 15 patients had normal RV, whereas 55 patients had RV dilatation (RVD) and/or RV systolic dysfunction (RVSD). Procollagens type I and III carboxy- and amino-terminal peptides, osteopontin (OPN), TGF1-b1, connective tissue growth factor (CTGF), MMP-2, MMP-9 and TIMP-1 were measured in serum as well as expression of miR-21, miR-26, miR-29, miR-30 and miR-133a. All patients underwent endomyocardial biopsy.

Results: Biopsy-proven fibrosis was evenly distributed in two groups. Serum levels of fibrosis markers did not differ between groups. OPN, CTGF, MMP-2, and TIMP-1 correlated with RV parameters. Only miR-133 a was differently expressed in both groups. MiR-21, miR-26, miR-30, and miR-133a cor­related with RV morphological but without functional parameters. Not a single marker of fibrosis was independently associated with RV. MiR-30 was associated with RV impairment in the logistic regression model adjusted for age, sex, body mass index, and disease duration; however, lost its significance in the more comprehensive model.

Conclusions: Right ventricle structural and functional abnormalities are common in DCM. ECM fibrosis and serum markers are not associated with RV impairment. The prognostic value of studied microRNAs on RV is limited in DCM.

Abstract

Background: The relationship between right ventricle (RV), extracellular matrix (ECM) fibrosis and fibrosis-linked, circulating microRNAs in dilated cardiomyopathy (DCM) is unknown.

Aim: The aim of the study was to uncover the associations between serum markers of ECM metabolism and circulating microRNAs with RV morphological and functional parameters.

Methods: The study population consisted of 70 consecutive DCM patients (ejection fraction 24.4 ± ± 7.4%). Based on detailed echocardiographic assessment — 15 patients had normal RV, whereas 55 patients had RV dilatation (RVD) and/or RV systolic dysfunction (RVSD). Procollagens type I and III carboxy- and amino-terminal peptides, osteopontin (OPN), TGF1-b1, connective tissue growth factor (CTGF), MMP-2, MMP-9 and TIMP-1 were measured in serum as well as expression of miR-21, miR-26, miR-29, miR-30 and miR-133a. All patients underwent endomyocardial biopsy.

Results: Biopsy-proven fibrosis was evenly distributed in two groups. Serum levels of fibrosis markers did not differ between groups. OPN, CTGF, MMP-2, and TIMP-1 correlated with RV parameters. Only miR-133 a was differently expressed in both groups. MiR-21, miR-26, miR-30, and miR-133a cor­related with RV morphological but without functional parameters. Not a single marker of fibrosis was independently associated with RV. MiR-30 was associated with RV impairment in the logistic regression model adjusted for age, sex, body mass index, and disease duration; however, lost its significance in the more comprehensive model.

Conclusions: Right ventricle structural and functional abnormalities are common in DCM. ECM fibrosis and serum markers are not associated with RV impairment. The prognostic value of studied microRNAs on RV is limited in DCM.

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Keywords

dilated cardiomyopathy, right ventricle, biopsy, extracellular matrix fibrosis, microRNA

About this article
Title

Right ventricular morphology and function is not related with microRNAs and fibrosis markers in dilated cardiomyopathy

Journal

Cardiology Journal

Issue

Vol 25, No 6 (2018)

Pages

722-731

Published online

2017-08-24

DOI

10.5603/CJ.a2017.0099

Pubmed

28840590

Bibliographic record

Cardiol J 2018;25(6):722-731.

Keywords

dilated cardiomyopathy
right ventricle
biopsy
extracellular matrix fibrosis
microRNA

Authors

Paweł Rubiś
Justyna Totoń-Żurańska
Sylwia Wiśniowska-Śmiałek
Maria Kołton-Wróż
Paweł Wołkow
Ewa Wypasek
Lucyna Rudnicka-Sosin
Agnieszka Pawlak
Kozanecki Kozanecki
Lidia Tomkiewicz-Pająk
Piotr Podolec

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