open access

Vol 25, No 2 (2018)
Original articles — Clinical cardiology
Published online: 2017-05-11
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Effect of fluid resuscitation with balanced solutions on platelets: In vitro simulation of 20% volume substitution

Łukasz J. Krzych, Piotr F. Czempik
DOI: 10.5603/CJ.a2017.0054
·
Pubmed: 28497841
·
Cardiol J 2018;25(2):254-259.

open access

Vol 25, No 2 (2018)
Original articles — Clinical cardiology
Published online: 2017-05-11

Abstract

 Background: Fluid resuscitation in massive bleeding may cause coagulation disorders by dilution of platelets and clotting factors or by the impact on their function. The aim of this study was to investigate the effects of balanced crystalloid and colloid solutions on platelets in vitro using complex assessment of coagulation.

Methods: The study group was comprised of 32 American Society of Anesthesiologists physical status class I male volunteers, aged 21–35 (29 ± 4) years, weighting 59–103 (81.2 ± 9.8) kg. Whole blood samples were diluted at a 4:1 ratio with the following fluids: balanced crystalloid (Plasmalyte®), 6% hydroxyethyl starch 130/0.4 (Volulyte®) and succinylated gelatin (Geloplasma®). Coagulation was as­sessed using standard morphology, rotational thromboelastometry and aggregometry.

Results: Dilution with all fluids caused statistically significant drop in the number of platelets (p < 0.01) but the effect did not differ between solutions (p > 0.05 for all). Other platelet parameters, such as platelet distribution width, mean platelet volume and platelet-large cell ratio were not affected by the solutions. Hemodilution had no effect on platelet function (p = 0.1). Decreased platelet component of clot strength was found for all three fluids (p < 0.05), although the effect for colloids was more pronounced.

Conclusions: The effect of balanced crystalloids and colloids on platelet aggregation was insignificant, even after 20% volume substitution with the resuscitation fluids. (

Abstract

 Background: Fluid resuscitation in massive bleeding may cause coagulation disorders by dilution of platelets and clotting factors or by the impact on their function. The aim of this study was to investigate the effects of balanced crystalloid and colloid solutions on platelets in vitro using complex assessment of coagulation.

Methods: The study group was comprised of 32 American Society of Anesthesiologists physical status class I male volunteers, aged 21–35 (29 ± 4) years, weighting 59–103 (81.2 ± 9.8) kg. Whole blood samples were diluted at a 4:1 ratio with the following fluids: balanced crystalloid (Plasmalyte®), 6% hydroxyethyl starch 130/0.4 (Volulyte®) and succinylated gelatin (Geloplasma®). Coagulation was as­sessed using standard morphology, rotational thromboelastometry and aggregometry.

Results: Dilution with all fluids caused statistically significant drop in the number of platelets (p < 0.01) but the effect did not differ between solutions (p > 0.05 for all). Other platelet parameters, such as platelet distribution width, mean platelet volume and platelet-large cell ratio were not affected by the solutions. Hemodilution had no effect on platelet function (p = 0.1). Decreased platelet component of clot strength was found for all three fluids (p < 0.05), although the effect for colloids was more pronounced.

Conclusions: The effect of balanced crystalloids and colloids on platelet aggregation was insignificant, even after 20% volume substitution with the resuscitation fluids. (

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Keywords

platelets, coagulopathy, rotational thromboelastometry, aggregometry, resuscitation fluids

About this article
Title

Effect of fluid resuscitation with balanced solutions on platelets: In vitro simulation of 20% volume substitution

Journal

Cardiology Journal

Issue

Vol 25, No 2 (2018)

Pages

254-259

Published online

2017-05-11

DOI

10.5603/CJ.a2017.0054

Pubmed

28497841

Bibliographic record

Cardiol J 2018;25(2):254-259.

Keywords

platelets
coagulopathy
rotational thromboelastometry
aggregometry
resuscitation fluids

Authors

Łukasz J. Krzych
Piotr F. Czempik

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