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Vol 22, No 4 (2018)
Original paper
Published online: 2018-11-29
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Blood lipids in patients with high and low physical activity depending on polymorphism of beta-2 and 3-adrenergic receptor genes

Anna Isayeva1, Maryna Vovchenko1, Anna Shalimova1, Valentina Halchynska1, Lyudmila Peteneva1, Tatiana Bondar1
·
Arterial Hypertension 2018;22(4):193-200.
Affiliations
  1. Department of Complex Risk Reduction of Chronic Non-Communicable Diseases, Government Institution L.T. Malaya Therapy National Institute of the National Academy of Medical Sciences of Ukraine, Kharkov, Ukraine

open access

Vol 22, No 4 (2018)
ORIGINAL PAPERS
Published online: 2018-11-29

Abstract

Background. The aim of the study was to investigate the lipid levels in patients with various physical activity and
polymorphous variants of ADRB2 (Gln27Glu and Arg16Gly) and ADRB3 (Trp64Arg) genes.

Material and methods. One hundred and fifty subjects were enrolled in the study; mean age was 44.7 ± 8.9 y.o.
Physical activity was assessed using Omron Walking style III step counter HJ-203-EK pedometer. Lipids were assessed
via enzymatic method using Cormay reagent kit (Poland). Genotyping of polymorphous sites of ADRB2
(Gln27Glu, rs1042714 and Arg16Gly, rs1042713) and ADRB3 (Trp64Agr, rs4994) genes was performed using
SNP-express-SHOT reagent kits manufactured by Litex Research and Production Company, via real-time polymerase
chain reaction.

Results. Individuals walking more than 5000 steps daily with Gly16Gly polymorphism had significantly higher
blood lipids. Total cholesterol (TC), triglycerides, LDL and HDL-cholesterol levels were 5.10 (3.86–5.74), 1.13
(0.66–1.51), 3.28 (2.14–3.70), 1.43 (1.32–1.63) mmol/L in Arg16 group and 5.55 (5.23–6.96), 1.56 (0.45–
–2.02), 3.87 (3.09–4.46) and 1.30 (1.13–1.68) mmol/L in Gly16Gly group. The differences in lipid parameters
were established for polymorphous variants of ADRB3 gene in patients walking more than 5000 steps daily. In
Trp54 carriers, TC, triglycerides, LDL and HDL-cholesterol levels were 5.23 (4.00–5.69), 1.25 (0.80–1.56), 3.25
(2.22–3.80) and 1.41 (1.26–1.53) mmol/L, respectively. In 54Arg carriers group, lipids were significantly higher:
TC 5.74 (5.59–6.88), triglycerides 1.51 (1.03–1.90), LDL-cholesterol 3.78 (3.38–5.06) and HDL-cholesterol
1.68 (1.13–1.81) mmol/L.

Conclusion. This analysis suggests that carriers of Arg16 of ADRB2 gene and/or Trp54 of ADRB3 gene tend to have
lower lipid parameters when they have high physical activity (walking more than 5000 steps a day) in comparison to
carriers of 16Gly and/or 54Agr. There is no association between lipids level and ADRB2 (Gln27Glu and Arg16Gly)
and ADRB3 (Trp64Agr) polymorphisms when individuals have low physical activity (walking less than 5000 steps
a day).

Abstract

Background. The aim of the study was to investigate the lipid levels in patients with various physical activity and
polymorphous variants of ADRB2 (Gln27Glu and Arg16Gly) and ADRB3 (Trp64Arg) genes.

Material and methods. One hundred and fifty subjects were enrolled in the study; mean age was 44.7 ± 8.9 y.o.
Physical activity was assessed using Omron Walking style III step counter HJ-203-EK pedometer. Lipids were assessed
via enzymatic method using Cormay reagent kit (Poland). Genotyping of polymorphous sites of ADRB2
(Gln27Glu, rs1042714 and Arg16Gly, rs1042713) and ADRB3 (Trp64Agr, rs4994) genes was performed using
SNP-express-SHOT reagent kits manufactured by Litex Research and Production Company, via real-time polymerase
chain reaction.

Results. Individuals walking more than 5000 steps daily with Gly16Gly polymorphism had significantly higher
blood lipids. Total cholesterol (TC), triglycerides, LDL and HDL-cholesterol levels were 5.10 (3.86–5.74), 1.13
(0.66–1.51), 3.28 (2.14–3.70), 1.43 (1.32–1.63) mmol/L in Arg16 group and 5.55 (5.23–6.96), 1.56 (0.45–
–2.02), 3.87 (3.09–4.46) and 1.30 (1.13–1.68) mmol/L in Gly16Gly group. The differences in lipid parameters
were established for polymorphous variants of ADRB3 gene in patients walking more than 5000 steps daily. In
Trp54 carriers, TC, triglycerides, LDL and HDL-cholesterol levels were 5.23 (4.00–5.69), 1.25 (0.80–1.56), 3.25
(2.22–3.80) and 1.41 (1.26–1.53) mmol/L, respectively. In 54Arg carriers group, lipids were significantly higher:
TC 5.74 (5.59–6.88), triglycerides 1.51 (1.03–1.90), LDL-cholesterol 3.78 (3.38–5.06) and HDL-cholesterol
1.68 (1.13–1.81) mmol/L.

Conclusion. This analysis suggests that carriers of Arg16 of ADRB2 gene and/or Trp54 of ADRB3 gene tend to have
lower lipid parameters when they have high physical activity (walking more than 5000 steps a day) in comparison to
carriers of 16Gly and/or 54Agr. There is no association between lipids level and ADRB2 (Gln27Glu and Arg16Gly)
and ADRB3 (Trp64Agr) polymorphisms when individuals have low physical activity (walking less than 5000 steps
a day).

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Keywords

blood lipids; physical activity; ADRB2 and ADRB3 genes polymorphisms

About this article
Title

Blood lipids in patients with high and low physical activity depending on polymorphism of beta-2 and 3-adrenergic receptor genes

Journal

Arterial Hypertension

Issue

Vol 22, No 4 (2018)

Article type

Original paper

Pages

193-200

Published online

2018-11-29

Page views

928

Article views/downloads

896

DOI

10.5603/AH.a2018.0020

Bibliographic record

Arterial Hypertension 2018;22(4):193-200.

Keywords

blood lipids
physical activity
ADRB2 and ADRB3 genes polymorphisms

Authors

Anna Isayeva
Maryna Vovchenko
Anna Shalimova
Valentina Halchynska
Lyudmila Peteneva
Tatiana Bondar

References (15)
  1. Zhang H, Wu J, Yu L. Association of Gln27Glu and Arg16Gly polymorphisms in Beta2-adrenergic receptor gene with obesity susceptibility: a meta-analysis. PLoS One. 2014; 9(6): e100489.
  2. Naka I, Hikami K, Nakayama K, et al. A functional SNP upstream of the beta-2 adrenergic receptor gene (ADRB2) is associated with obesity in Oceanic populations. Int J Obes (Lond). 2013; 37(9): 1204–1210.
  3. Baturin AK, Sorokina EIu, Pogozheva AV, et al. [Regional features of obesity-associated gene polymorphism (rs9939609 FTO gene and gene Trp64Arg ADRB3) in Russian population]. Vopr Pitan. 2014; 83(2): 35–41.
  4. Pereira AC, Floriano MS, Mota GFA, et al. Beta2 adrenoceptor functional gene variants, obesity, and blood pressure level interactions in the general population. Hypertension. 2003; 42(4): 685–692.
  5. Daghestani M, Daghestani M, Daghistani M, et al. ADRB3 polymorphism rs4994 (Trp64Arg) associates significantly with bodyweight elevation and dyslipidaemias in Saudis but not rs1801253 (Arg389Gly) polymorphism in ARDB1. Lipids Health Dis. 2018; 17(1): 58.
  6. Maia-Landim A, Ramírez JM, Lancho C, et al. Long-term effects of Garcinia cambogia/Glucomannan on weight loss in people with obesity, PLIN4, FTO and Trp64Arg polymorphisms. BMC Complement Altern Med. 2018; 18(1): 26.
  7. Szendrei B, González-Lamuño D, Amigo T, et al. PRONAF Study Group. Influence of ADRB2 Gln27Glu and ADRB3 Trp64Arg polymorphisms on body weight and body composition changes after a controlled weight-loss intervention. Appl Physiol Nutr Metab. 2016; 41(3): 307–314.
  8. Chen Y, Wang X, Shen Z, et al. Effect of the beta-3 adrenergic receptor Trp64Arg and uncoupling protein 1-3826 A>G genotypes on lipid and apolipoprotein levels in overweight/obese and non-obese Chinese subjects. Lipids Health Dis. 2015; 14: 34.
  9. Miyaki K, Sutani S, Kikuchi H, et al. Increased risk of obesity resulting from the interaction between high energy intake and the Trp64Arg polymorphism of the beta3-adrenergic receptor gene in healthy Japanese men. J Epidemiol. 2005; 15(6): 203–210.
  10. Petrone A, Zavarella S, Iacobellis G, et al. Association of beta2 adrenergic receptor polymorphisms and related haplotypes with triglyceride and LDL-cholesterol levels. Eur J Hum Genet. 2006; 14(1): 94–100.
  11. Tomaszewski M, Charchar FJ, Lacka B, et al. Epistatic interaction between beta2-adrenergic receptor and neuropeptide Y genes influences LDL-cholesterol in hypertension. Hypertension. 2004; 44(5): 689–694.
  12. Ramos-Lopez O, Riezu-Boj JI, Milagro FI, et al. Differential lipid metabolism outcomes associated with ADRB2 gene polymorphisms in response to two dietary interventions in overweight/obese subjects. Nutr Metab Cardiovasc Dis. 2018; 28(2): 165–172.
  13. Brondani LA, Duarte GCK, Canani LH, et al. The presence of at least three alleles of the ADRB3 Trp64Arg (C/T) and UCP1 –3826A/G polymorphisms is associated with protection to overweight/obesity and with higher high-density lipoprotein cholesterol levels in Caucasian-Brazilian patients with type 2 diabetes. Metab Syndr Relat Disord. 2014; 12(1): 16–24.
  14. Ryuk JAh, Zhang X, Ko BS, et al. Association of β3-adrenergic receptor rs4994 polymorphisms with the risk of type 2 diabetes: A systematic review and meta-analysis. Diabetes Res Clin Pract. 2017; 129: 86–96.
  15. Leońska-Duniec A, Jastrzębski Z, Jażdżewska A, et al. Individual Responsiveness to Exercise-Induced Fat Loss and Improvement of Metabolic Profile in Young Women is Associated with Polymorphisms of Adrenergic Receptor Genes. J Sports Sci Med. 2018; 17(1): 134–144.

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