open access

Vol 88, No 4 (2020)
ORIGINAL PAPERS
Published online: 2020-07-15
Submitted: 2020-02-17
Accepted: 2020-04-12
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Are level of IL-13 and IL-4 predictive for formation of chronic inflammation in children with asthma?

Nataliia Makieieva, Valeriia Malakhova, Yuliia Vasylchenko, Valentyn Tsymbal
DOI: 10.5603/ARM.a2020.0108
·
Pubmed: 32869265
·
Adv Respir Med 2020;88(4):320-326.

open access

Vol 88, No 4 (2020)
ORIGINAL PAPERS
Published online: 2020-07-15
Submitted: 2020-02-17
Accepted: 2020-04-12

Abstract

Introduction: Asthma diagnosis in young children may represent a clinical challenge. There are no standard prognostic and dia-gnostic methods. The aim of the study was to evaluate the clinical and prognostic assessment of IL-4 and IL-13 concentrations in children with recurrent wheezing.
Material and methods: The study included 96 children with recurrent wheezing. 81 patients were diagnosed as transient wheezing, 15 patients with asthma, and 25 healthy children were selected as controls. The concentrations of IL-4 and IL-13 were analyzed in exhaled breath condensate (EBC) using an enzyme-linked immunosorbent assay (ELISA). Data analysis was performed using Statsoft Statistica Version 8 (Tulsa, OK) and the statistical program MedCalc version 17.2.
Results: Both IL-4 and IL-13 concentrations were significantly higher in DDA (21.13 pg/mL, 26.13 pg/mL, respectively) and TW (13.86 pg/mL, 18.3 pg/mL, respectively) groups as compared to healthy controls (3.37 pg/mL, 16.35 pg/mL, respectively; p < 0.001), and the highest rates were observed in children with diagnosed asthma (p < 0.001, DDW vs TW, respectively). IL-4 concentration higher than 18.45 pg/mL (with sensitivity 86.7% and specificity 80%) and IL-13 concentration higher than 20.17 pg/ /mL (with sensitivity 100% and specificity 76.7%) in EBC in children with wheezing recurrence can be considered as a possible predictor of asthma development.
Conclusions: The concentration of the anti-inflammatory cytokines IL-4 and IL-13 were significantly increased in children with recurrent wheezing and the highest rates were found in asthma developing children. The concentrations of IL-4 and IL-13 in chil-dren with wheezing can be considered as a possible predictor of asthma development.

Abstract

Introduction: Asthma diagnosis in young children may represent a clinical challenge. There are no standard prognostic and dia-gnostic methods. The aim of the study was to evaluate the clinical and prognostic assessment of IL-4 and IL-13 concentrations in children with recurrent wheezing.
Material and methods: The study included 96 children with recurrent wheezing. 81 patients were diagnosed as transient wheezing, 15 patients with asthma, and 25 healthy children were selected as controls. The concentrations of IL-4 and IL-13 were analyzed in exhaled breath condensate (EBC) using an enzyme-linked immunosorbent assay (ELISA). Data analysis was performed using Statsoft Statistica Version 8 (Tulsa, OK) and the statistical program MedCalc version 17.2.
Results: Both IL-4 and IL-13 concentrations were significantly higher in DDA (21.13 pg/mL, 26.13 pg/mL, respectively) and TW (13.86 pg/mL, 18.3 pg/mL, respectively) groups as compared to healthy controls (3.37 pg/mL, 16.35 pg/mL, respectively; p < 0.001), and the highest rates were observed in children with diagnosed asthma (p < 0.001, DDW vs TW, respectively). IL-4 concentration higher than 18.45 pg/mL (with sensitivity 86.7% and specificity 80%) and IL-13 concentration higher than 20.17 pg/ /mL (with sensitivity 100% and specificity 76.7%) in EBC in children with wheezing recurrence can be considered as a possible predictor of asthma development.
Conclusions: The concentration of the anti-inflammatory cytokines IL-4 and IL-13 were significantly increased in children with recurrent wheezing and the highest rates were found in asthma developing children. The concentrations of IL-4 and IL-13 in chil-dren with wheezing can be considered as a possible predictor of asthma development.

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Keywords

asthma; inflammation; IL-4; IL-13; children

About this article
Title

Are level of IL-13 and IL-4 predictive for formation of chronic inflammation in children with asthma?

Journal

Advances in Respiratory Medicine

Issue

Vol 88, No 4 (2020)

Pages

320-326

Published online

2020-07-15

DOI

10.5603/ARM.a2020.0108

Pubmed

32869265

Bibliographic record

Adv Respir Med 2020;88(4):320-326.

Keywords

asthma
inflammation
IL-4
IL-13
children

Authors

Nataliia Makieieva
Valeriia Malakhova
Yuliia Vasylchenko
Valentyn Tsymbal

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