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The TNF-α, P53 protein response and lung respiratory changes to exercise, chronic hypoxia and Adiantum capillus-veneris supplementation
Affiliations- Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Mazandaran, Iran
- Faculty of Aerospace Medicine and subsurface, Army Medical University, Tehran, Iran
- Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
- Colonel Physician, Army Ground Forces, Health Relief and Treatment Center, Teheran, Iran
- Department of Biological Sciences in Sport, Faculty of Sports Sciences and Health, Shahid Beheshti University, Teheran, Iran
- Université de Rennes, M2S, Laboratoire Mouvement, Sport, Santé, Rennes, France
- Higher Institutes of Sport and Physical Education of Ksar-Said, Ksar-Said, Tunisia.
- Department of Exercise & Sport Science, University of North Carolina, Chapel Hill, NC, USA.
open access
Abstract
Introduction: Evidence suggests that hypoxia and high-intensity exercise training can increase apoptosis of lung cells and Adiantum capillus-veneris (Ac-v) extract can have anti-apoptotic effects. Thus, the aim of the present study was to investigate the effect of chronic hypoxia and the (Ac-v) extraction as a supplement on TNF-a and P53 protein expression as well as the respiratory surface. Material and methods: 24 healthy Wistar rats (age = 4 weeks, weight = 72 = 9 gr) were trained using interval training for 6 weeks followed by a 3-week stay in hypoxia conditions. Half of the hypoxia samples received 500 ml/gr/per body weight daily (Ac-v) within 3 weeks of hypoxia. At the end, the lung tissue was removed for histological and immunohistological analysis. Results: After 3 weeks of hypoxia exposure following 6 weeks of exercise, expression of P53 and TNF-a increased and the respiratory surface decreased (p ≤ 0.05). After 3 weeks of taking the Ac-v extract during hypoxia exposure, reduced P53 and TNF-a expression and the increased respiratory surface were observed (p ≤ 0.05). Conclusions: Chronic hypoxia may be considered as a strong stimulus leading to the expression of proteins involved in apoptosis and tissue disruption. However, our findings suggest that the antioxidative properties of Ac-v extract could decrease the destructive structural and molecular events that happen along with hypoxia exposure or intense exercise training.
Abstract
Introduction: Evidence suggests that hypoxia and high-intensity exercise training can increase apoptosis of lung cells and Adiantum capillus-veneris (Ac-v) extract can have anti-apoptotic effects. Thus, the aim of the present study was to investigate the effect of chronic hypoxia and the (Ac-v) extraction as a supplement on TNF-a and P53 protein expression as well as the respiratory surface. Material and methods: 24 healthy Wistar rats (age = 4 weeks, weight = 72 = 9 gr) were trained using interval training for 6 weeks followed by a 3-week stay in hypoxia conditions. Half of the hypoxia samples received 500 ml/gr/per body weight daily (Ac-v) within 3 weeks of hypoxia. At the end, the lung tissue was removed for histological and immunohistological analysis. Results: After 3 weeks of hypoxia exposure following 6 weeks of exercise, expression of P53 and TNF-a increased and the respiratory surface decreased (p ≤ 0.05). After 3 weeks of taking the Ac-v extract during hypoxia exposure, reduced P53 and TNF-a expression and the increased respiratory surface were observed (p ≤ 0.05). Conclusions: Chronic hypoxia may be considered as a strong stimulus leading to the expression of proteins involved in apoptosis and tissue disruption. However, our findings suggest that the antioxidative properties of Ac-v extract could decrease the destructive structural and molecular events that happen along with hypoxia exposure or intense exercise training.
Keywords
apoptosis, lung, interval training, respiratory surface
About this articleTitle
The TNF-α, P53 protein response and lung respiratory changes to exercise, chronic hypoxia and Adiantum capillus-veneris supplementation
Journal
Advances in Respiratory Medicine
Issue
Article type
Research paper
Pages
226-234
Published online
2019-08-30
Page views
1159
Article views/downloads
1103
DOI
10.5603/ARM.2019.0037
Pubmed
Bibliographic record
Adv Respir Med 2019;87(4):226-234.
Keywords
apoptosis
lung
interval training
respiratory surface
Authors
Mehdi Yadegari
Simin Riahy
Shadmehr Mirdar
Gholamreza Hamidian
Seyed Mohsen Afkhami
Ayoub Saeidi
Fatma Rhibi
Abderraouf Ben Abderrahman
Anthony C. Hackney
Hassane Zouhal
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