open access
Vol 85, No 6 (2017)
Research paper
Published online: 2017-12-14
Submitted: 2017-10-20
Accepted: 2017-11-26
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Histone deacetylases affect transcriptional regulation of CCL2 and CXCL8 expression by pulmonary fibroblasts in vitro
Paweł Golec, Paweł Leszek Bernatowicz, Grażyna Tokajuk, Mirosław Kozłowski, Krzysztof Kowal
open access
Vol 85, No 6 (2017)
ORIGINAL PAPERS
Published online: 2017-12-14
Submitted: 2017-10-20
Accepted: 2017-11-26
Abstract
Introduction: Chemokines have been shown to play an important role in tissue remodeling and fibrosis in the respiratory system. In this
study we wanted to evaluate the mechanisms, which regulate the expression of selected chemokines by pulmonary fibroblasts in vitro.
Material and methods: Pulmonary fibroblasts were cultured with and without bacterial lipopolysaccharide
(LPS) for 6 hours. In addition some of the cultures were pre-treated with histone deacetylase inhibitor Trichostatin
A (TSA). Real-time PCR reaction was performed to estimate the expression of chemokines CCL2, CCL3 and CXCL8.
Results: In unstimulated cultures detectable expression of CCL2 and CXCL8 was observed, while CCL3
expression could not be detected. After stimulation with LPS, TSA and both agents together CCL2 expression
rose by 1.52, 1.62 and 1.8 times in comparison to control cultures respectively. CXCL8 mRNA expression
levels after stimulation with LPS, TSA and LPSTSA increased by 1.53, 1.91 and 2.4 times accordingly.
Conclusion: Epigenetic mechanisms related to histone acetylation affects transcriptional regulation of CCL2 and CXCL8 expression
by pulmonary fibroblasts. Those mechanisms may play a role in tissue repair and pathologic remodeling.
Abstract
Introduction: Chemokines have been shown to play an important role in tissue remodeling and fibrosis in the respiratory system. In this
study we wanted to evaluate the mechanisms, which regulate the expression of selected chemokines by pulmonary fibroblasts in vitro.
Material and methods: Pulmonary fibroblasts were cultured with and without bacterial lipopolysaccharide
(LPS) for 6 hours. In addition some of the cultures were pre-treated with histone deacetylase inhibitor Trichostatin
A (TSA). Real-time PCR reaction was performed to estimate the expression of chemokines CCL2, CCL3 and CXCL8.
Results: In unstimulated cultures detectable expression of CCL2 and CXCL8 was observed, while CCL3
expression could not be detected. After stimulation with LPS, TSA and both agents together CCL2 expression
rose by 1.52, 1.62 and 1.8 times in comparison to control cultures respectively. CXCL8 mRNA expression
levels after stimulation with LPS, TSA and LPSTSA increased by 1.53, 1.91 and 2.4 times accordingly.
Conclusion: Epigenetic mechanisms related to histone acetylation affects transcriptional regulation of CCL2 and CXCL8 expression
by pulmonary fibroblasts. Those mechanisms may play a role in tissue repair and pathologic remodeling.
Get Citation
Keywords
CCL2, CXCL8, histone deacetylase inhibitors, fibroblasts, real-time polymerase chain reaction
About this articleTitle
Histone deacetylases affect transcriptional regulation of CCL2 and CXCL8 expression by pulmonary fibroblasts in vitro
Journal
Advances in Respiratory Medicine
Issue
Article type
Research paper
Pages
307-312
Published online
2017-12-14
DOI
10.5603/ARM.2017.0053
Pubmed
Bibliographic record
Adv Respir Med 2017;85(6):307-312.
Keywords
CCL2
CXCL8
histone deacetylase inhibitors
fibroblasts
real-time polymerase chain reaction
Authors
Paweł Golec
Paweł Leszek Bernatowicz
Grażyna Tokajuk
Mirosław Kozłowski
Krzysztof Kowal
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