open access

Vol 85, No 1 (2017)
ORIGINAL PAPERS
Submitted: 2017-02-13
Accepted: 2017-02-13
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The content of mucin MUC-2, -3 and -4 antigens in the bronchial mucosa membrane of chronic obstructive pulmonary disease patients during acute exacerbation — initial report

Svetlana Kovalenko, Andrey Dorofieiev
DOI: 10.5603/ARM.2017.0002
·
Adv Respir Med 2017;85(1):3-7.

open access

Vol 85, No 1 (2017)
ORIGINAL PAPERS
Submitted: 2017-02-13
Accepted: 2017-02-13

Abstract

INTRODUCTION: Changes in mucin production and dyscrinia are common features of inflammation in chronic obstructive pulmonary disease (COPD). Immunohistochemical assessment of MUC-2, MUC-3, MUC-4 expression in the integumentary epithelium, goblet cells, the epithelium of mucous glands and stroma fusiform cells of the bronchial mucosa of COPD patients during an infectious and noninfectious exacerbation was performed.

MATERIAL AND METHODS: 30 patients with stage III COPD were enrolled to the study. Patients were divided into 2 groups: group A — 14 patients with non-infectious acute exacerbation of COPD (AECOPD) and group B — 16 patients with infectious AECOPD. Fiberoptic bronchoscopy (FBS) and in vivo bronchial biopsy of bronchial mucosa were implemented to determine the extent and nature of bronchial inflammation. The optical density of specific color in bronchial structures was assessed using immunohistochemical staining to MUC-2, -3 and -4 antigens by means of primary monoclonal antibodies to these proteins, and visualization system Dako EnVision + System, Peroxidase (AEC).

RESULTS AND CONCLUSIONS: We detected that in different types of bronchial mucosa epithelial cells, during acute infectious exacerbation, a decrease of antigens MUC-2 and MUC-3 expression of a various degree may occur. This phenomenon in the stroma fusiform cells in AECOPD may be a sign of epithelial-mesenchymal transition, that may play a role in the development of an inflammatory process and progression of fibrosis in COPD.

Abstract

INTRODUCTION: Changes in mucin production and dyscrinia are common features of inflammation in chronic obstructive pulmonary disease (COPD). Immunohistochemical assessment of MUC-2, MUC-3, MUC-4 expression in the integumentary epithelium, goblet cells, the epithelium of mucous glands and stroma fusiform cells of the bronchial mucosa of COPD patients during an infectious and noninfectious exacerbation was performed.

MATERIAL AND METHODS: 30 patients with stage III COPD were enrolled to the study. Patients were divided into 2 groups: group A — 14 patients with non-infectious acute exacerbation of COPD (AECOPD) and group B — 16 patients with infectious AECOPD. Fiberoptic bronchoscopy (FBS) and in vivo bronchial biopsy of bronchial mucosa were implemented to determine the extent and nature of bronchial inflammation. The optical density of specific color in bronchial structures was assessed using immunohistochemical staining to MUC-2, -3 and -4 antigens by means of primary monoclonal antibodies to these proteins, and visualization system Dako EnVision + System, Peroxidase (AEC).

RESULTS AND CONCLUSIONS: We detected that in different types of bronchial mucosa epithelial cells, during acute infectious exacerbation, a decrease of antigens MUC-2 and MUC-3 expression of a various degree may occur. This phenomenon in the stroma fusiform cells in AECOPD may be a sign of epithelial-mesenchymal transition, that may play a role in the development of an inflammatory process and progression of fibrosis in COPD.

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Keywords

mucins, bronchial mucosa, inflammation

About this article
Title

The content of mucin MUC-2, -3 and -4 antigens in the bronchial mucosa membrane of chronic obstructive pulmonary disease patients during acute exacerbation — initial report

Journal

Advances in Respiratory Medicine

Issue

Vol 85, No 1 (2017)

Pages

3-7

DOI

10.5603/ARM.2017.0002

Bibliographic record

Adv Respir Med 2017;85(1):3-7.

Keywords

mucins
bronchial mucosa
inflammation

Authors

Svetlana Kovalenko
Andrey Dorofieiev

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