open access

Vol 84, No 1 (2016)
ORIGINAL PAPERS
Published online: 2015-11-24
Submitted: 2015-08-02
Accepted: 2015-10-30
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Serum levels of apoptosis-related markers (sFasL, TNF-a, p53 and bcl-2) in COPD patients

Monika Kosacka, Irena Porębska, Anna Korzeniewska, Renata Rubinsztajn, Marcin Grabicki, Renata Jankowska, Halina Batura-Gabryel, Ryszarda Chazan
DOI: 10.5603/PiAP.a2015.0080
·
Pneumonol Alergol Pol 2016;84(1):11-15.

open access

Vol 84, No 1 (2016)
ORIGINAL PAPERS
Published online: 2015-11-24
Submitted: 2015-08-02
Accepted: 2015-10-30

Abstract

Introduction: Taking into account important role of apoptosis in COPD pathogenesis, we wanted to asses the serum levels of markers involved in apoptosis regulation, including apoptosis inducers such as TNF-a, sFasL or p53 protein and apoptosis inhibitor bcl-2 and, in addition, to compare these markers with selected COPD parameters.

Material and methods: In 181 patients (60 women) with COPD (age was 62.2+ 9.37 years; FEV1% 55.2 + 19.98 %) and in 29 controls (11 women), serum levels of TNF-a, sFasL, p53 and bcl-2 were evaluated by the enzyme-linked immunosorbent assay (ELISA) method.

Results: In COPD patients the mean sFasL level was 0.092 ± 0.077 ng/ml and mean TNF-a level was 2.911 ± 3.239 pg/ml. There were no differences in serum sFasL and TNF-a in COPD patients and control group. TNF-a and sFasL did not correlate with COPD parameters such as FEV1%, BMI, RV% (percentage of predicted value of residual volume) or BODE. Although we tried to evaluate bcl-2 and p53 protein serum levels with two different tests, measurable levels of bcl-2 were only detected in 15 patients and p53 in only 3 patients. Bcl-2 values were from 0.418 to 11.423 ng/ml and p53 from 90.772 to 994.749 pg/ml.

Conclusions: We didn’t observe any differences in serum levels of pro- and antiapoptotic markers in COPD patients and the control group or correlations between the markers studied and COPD parameters.

Abstract

Introduction: Taking into account important role of apoptosis in COPD pathogenesis, we wanted to asses the serum levels of markers involved in apoptosis regulation, including apoptosis inducers such as TNF-a, sFasL or p53 protein and apoptosis inhibitor bcl-2 and, in addition, to compare these markers with selected COPD parameters.

Material and methods: In 181 patients (60 women) with COPD (age was 62.2+ 9.37 years; FEV1% 55.2 + 19.98 %) and in 29 controls (11 women), serum levels of TNF-a, sFasL, p53 and bcl-2 were evaluated by the enzyme-linked immunosorbent assay (ELISA) method.

Results: In COPD patients the mean sFasL level was 0.092 ± 0.077 ng/ml and mean TNF-a level was 2.911 ± 3.239 pg/ml. There were no differences in serum sFasL and TNF-a in COPD patients and control group. TNF-a and sFasL did not correlate with COPD parameters such as FEV1%, BMI, RV% (percentage of predicted value of residual volume) or BODE. Although we tried to evaluate bcl-2 and p53 protein serum levels with two different tests, measurable levels of bcl-2 were only detected in 15 patients and p53 in only 3 patients. Bcl-2 values were from 0.418 to 11.423 ng/ml and p53 from 90.772 to 994.749 pg/ml.

Conclusions: We didn’t observe any differences in serum levels of pro- and antiapoptotic markers in COPD patients and the control group or correlations between the markers studied and COPD parameters.

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Keywords

apoptosis, bcl-2, COPD, p53, sFasL, TNF-a

About this article
Title

Serum levels of apoptosis-related markers (sFasL, TNF-a, p53 and bcl-2) in COPD patients

Journal

Advances in Respiratory Medicine

Issue

Vol 84, No 1 (2016)

Pages

11-15

Published online

2015-11-24

DOI

10.5603/PiAP.a2015.0080

Bibliographic record

Pneumonol Alergol Pol 2016;84(1):11-15.

Keywords

apoptosis
bcl-2
COPD
p53
sFasL
TNF-a

Authors

Monika Kosacka
Irena Porębska
Anna Korzeniewska
Renata Rubinsztajn
Marcin Grabicki
Renata Jankowska
Halina Batura-Gabryel
Ryszarda Chazan

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