open access

Vol 83, No 1 (2015)
REVIEWS
Submitted: 2015-01-08
Accepted: 2015-01-08
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Application of basophil activation test in diagnosing aspirin hypersensitivity

Marika Gawinowska, Krzysztof Specjalski, Marta Chełmińska, Jakub Łata, Maciej Zieliński
DOI: 10.5603/PiAP.2015.0010
·
Pneumonol Alergol Pol 2015;83(1):66-73.

open access

Vol 83, No 1 (2015)
REVIEWS
Submitted: 2015-01-08
Accepted: 2015-01-08

Abstract

In the face of increasing prevalence of hypersensitivity reactions, introduction of effective, reliable and safe methods plays a crucial role in their diagnosing. Among the currently available laboratory (in vitro) methods is basophil activation test (BAT). It is a flow- cytometry based assay that allows to identificate in the blood sample basophils and additionally to asses the degree of cell activation after exposure to an antigen. The most common superficial identification markers are CD63 and CD203c, which increase in number after activation. Basophil actvation test can be applied to confirm diagnosis of allergy to Hymenoptera venoms, food, pollens and hypersensitivity to drugs. The aim of present paper is to present theoretical methods of this test as well as its pros and cons. We focus also on presentation of clinical case where BAT seemed to be a necessary addition to a routine diagnostic pathway. We present a case of identification of the culprit drug which caused an anaphylactic reaction.

Abstract

In the face of increasing prevalence of hypersensitivity reactions, introduction of effective, reliable and safe methods plays a crucial role in their diagnosing. Among the currently available laboratory (in vitro) methods is basophil activation test (BAT). It is a flow- cytometry based assay that allows to identificate in the blood sample basophils and additionally to asses the degree of cell activation after exposure to an antigen. The most common superficial identification markers are CD63 and CD203c, which increase in number after activation. Basophil actvation test can be applied to confirm diagnosis of allergy to Hymenoptera venoms, food, pollens and hypersensitivity to drugs. The aim of present paper is to present theoretical methods of this test as well as its pros and cons. We focus also on presentation of clinical case where BAT seemed to be a necessary addition to a routine diagnostic pathway. We present a case of identification of the culprit drug which caused an anaphylactic reaction.

Get Citation

Keywords

basophil activation test, BAT, hypersensitivity, NSAIDs, ASA

About this article
Title

Application of basophil activation test in diagnosing aspirin hypersensitivity

Journal

Advances in Respiratory Medicine

Issue

Vol 83, No 1 (2015)

Pages

66-73

DOI

10.5603/PiAP.2015.0010

Bibliographic record

Pneumonol Alergol Pol 2015;83(1):66-73.

Keywords

basophil activation test
BAT
hypersensitivity
NSAIDs
ASA

Authors

Marika Gawinowska
Krzysztof Specjalski
Marta Chełmińska
Jakub Łata
Maciej Zieliński

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