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Vol 72, No 3-4 (2004)
Research paper
Submitted: 2013-02-22
Published online: 2008-02-18
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Antimycobacterial antibody level in pleural, pericardial and cerebrospinal fluid of patients with tuberculosis

Urszula Demkow, Małgorzata Filewska, Beata Białas, Monika Szturmowicz, Tadeusz Zielonka, Stefan Wesołowski, Jan Kuś, Jerzy Ziółkowski, Ewa Augustynowicz-Kopeć, ZofiaZwolska, Ewa Skopińska-Różewska, Ewa Rowińska-Zakrzewska
Pneumonol Alergol Pol 2004;72(3-4):105-110.

open access

Vol 72, No 3-4 (2004)
ORIGINAL PAPERS
Submitted: 2013-02-22
Published online: 2008-02-18

Abstract

The goal of the study was to evaluate IgG, IgA and IgM mediated humoral immune response against 38kDa and 16 kDa or 38kDa and LAM mycobacterial antigens in pleural, pericardial or cerebrospinal fluidfrompatients with tuberculosis (TB) and to compare to non-tuberculous controls (NTB). 30 cerebrospinal fluids(CSF)(16 TB pts and 14 NTB pts),17 pericardial fluids(6 TB and 11 NTB)and 20 pleural fluids(7 TB and 13 NTB) were examined. Commercially available ELISA-based as says (Pathozyme Tb complex plus, Myco G, A and M - Omega Diagnostic) were used. Tests were performed and cut off established according to manufacturer instruction. Mean IgG level against 38 + 16kDa was significantly higher in neurotuberculosis group compared to control (p < 0.05). Sensitivity of the test in detecting neurotuberculosis was of 42% and specificity of 96%. Mean IgG, IgA and IgM against 38kDa + LAM level was higher in TB group compared to NTB in CSF. No difference was observed between TB and NTB group in pleural effusion. Antimycobacterial antibody levels were non-significantly increased in pericardial fluid in TB. The findings of the study indicate that TB is associatedwiththe presence of detectable levels of antibodies in the CSF and pericardial effusion. Anti 38kDa + 16kDa IgG test can be used in combination with other diagnostic methods to increase diagnostic accuracy of neurotuberculosis.

Abstract

The goal of the study was to evaluate IgG, IgA and IgM mediated humoral immune response against 38kDa and 16 kDa or 38kDa and LAM mycobacterial antigens in pleural, pericardial or cerebrospinal fluidfrompatients with tuberculosis (TB) and to compare to non-tuberculous controls (NTB). 30 cerebrospinal fluids(CSF)(16 TB pts and 14 NTB pts),17 pericardial fluids(6 TB and 11 NTB)and 20 pleural fluids(7 TB and 13 NTB) were examined. Commercially available ELISA-based as says (Pathozyme Tb complex plus, Myco G, A and M - Omega Diagnostic) were used. Tests were performed and cut off established according to manufacturer instruction. Mean IgG level against 38 + 16kDa was significantly higher in neurotuberculosis group compared to control (p < 0.05). Sensitivity of the test in detecting neurotuberculosis was of 42% and specificity of 96%. Mean IgG, IgA and IgM against 38kDa + LAM level was higher in TB group compared to NTB in CSF. No difference was observed between TB and NTB group in pleural effusion. Antimycobacterial antibody levels were non-significantly increased in pericardial fluid in TB. The findings of the study indicate that TB is associatedwiththe presence of detectable levels of antibodies in the CSF and pericardial effusion. Anti 38kDa + 16kDa IgG test can be used in combination with other diagnostic methods to increase diagnostic accuracy of neurotuberculosis.

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Keywords

Pleural tuberculosis; pericardial tuberculosis; neurotuberculosis; humoral immune response

About this article
Title

Antimycobacterial antibody level in pleural, pericardial and cerebrospinal fluid of patients with tuberculosis

Journal

Advances in Respiratory Medicine

Issue

Vol 72, No 3-4 (2004)

Article type

Research paper

Pages

105-110

Published online

2008-02-18

Bibliographic record

Pneumonol Alergol Pol 2004;72(3-4):105-110.

Keywords

Pleural tuberculosis
pericardial tuberculosis
neurotuberculosis
humoral immune response

Authors

Urszula Demkow
Małgorzata Filewska
Beata Białas
Monika Szturmowicz
Tadeusz Zielonka
Stefan Wesołowski
Jan Kuś
Jerzy Ziółkowski
Ewa Augustynowicz-Kopeć
ZofiaZwolska
Ewa Skopińska-Różewska
Ewa Rowińska-Zakrzewska

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