open access

Vol 72, No 5-6 (2004)
ORIGINAL PAPERS
Published online: 2008-02-18
Submitted: 2013-02-22
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IgG anti-multiantigen sonicate of mycobacterium tuberculosis (Mts) measured by ELISA-Mts

Agnieszka Bonar, Magdalena Chmiela, Barbara Różalska
Pneumonol Alergol Pol 2004;72(5-6):206-210.

open access

Vol 72, No 5-6 (2004)
ORIGINAL PAPERS
Published online: 2008-02-18
Submitted: 2013-02-22

Abstract

Laboratory diagnosis of tuberculosis (TB) is often difficult. In recent years the detection of TB bys erologic means has been the subject of a number of investigations.We studied the diagnostic utility of enzyme-linked immunosorbent assay in pulmonary tuberculosis. Two serological tests, commercial Pathozyme-Myco IgG (Omega Diagn.), and self-prepared ELISA-Mts using mycobacteria sonicate were evaluated simultaneously with 132 serum samples. Frequency and level of anti-mycobacterial IgG were compared in sera from 68 patients with TB and in sera from 64 healthy volunteers. The sensitivities of the tests with sera from TB patients were, respectively 55.9% and 70.6%. The specificities of these tests were respectively 98.5%and 84.4%. By combining two tests, a maximum sensitivity of 76.5% was obtained with reciprocal drops in specificity to 82.8%. Despite of that, it has been suggested that combinations of different antigens were attempted in order to improve the diagnostic yield.

Abstract

Laboratory diagnosis of tuberculosis (TB) is often difficult. In recent years the detection of TB bys erologic means has been the subject of a number of investigations.We studied the diagnostic utility of enzyme-linked immunosorbent assay in pulmonary tuberculosis. Two serological tests, commercial Pathozyme-Myco IgG (Omega Diagn.), and self-prepared ELISA-Mts using mycobacteria sonicate were evaluated simultaneously with 132 serum samples. Frequency and level of anti-mycobacterial IgG were compared in sera from 68 patients with TB and in sera from 64 healthy volunteers. The sensitivities of the tests with sera from TB patients were, respectively 55.9% and 70.6%. The specificities of these tests were respectively 98.5%and 84.4%. By combining two tests, a maximum sensitivity of 76.5% was obtained with reciprocal drops in specificity to 82.8%. Despite of that, it has been suggested that combinations of different antigens were attempted in order to improve the diagnostic yield.
Get Citation

Keywords

pulmonary tuberculosis; IgG ELISA tests; 38 kDa+LAM; M. tuberculosis sonicate antigens

About this article
Title

IgG anti-multiantigen sonicate of mycobacterium tuberculosis (Mts) measured by ELISA-Mts

Journal

Advances in Respiratory Medicine

Issue

Vol 72, No 5-6 (2004)

Pages

206-210

Published online

2008-02-18

Bibliographic record

Pneumonol Alergol Pol 2004;72(5-6):206-210.

Keywords

pulmonary tuberculosis
IgG ELISA tests
38 kDa+LAM
M. tuberculosis sonicate antigens

Authors

Agnieszka Bonar
Magdalena Chmiela
Barbara Różalska

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