Vol 74, No 1 (2006)
ORIGINAL PAPERS
Published online: 2008-02-18
Submitted: 2013-02-22
Macrophage phenotype in induced sputum in asthma subjects
Joanna Hermanowicz-Salamon, Joanna Domagała-Kulawik, Marta Maskey-Warzęchowska, Ryszarda Chazan
Pneumonol Alergol Pol 2006;74(1):101-105.
Vol 74, No 1 (2006)
ORIGINAL PAPERS
Published online: 2008-02-18
Submitted: 2013-02-22
Abstract
Macrophages represent the most predominant immune effector cells in the alveolar spaces and conducting
airways and are known to express activated phenotype. The study was aimed at assessing the differences in cellular profile and the expression of selected surface markers on sputum macrophages in asthma and healthy subjects.
17 healthy subjects (never smoked) and 10 mild asthma subjects treated with glucocorticosteroids were enrolled into the study. For macrophage phenotyping a immunocytochemistry method was used with commercially available
antibodies anti: CD14, CD71, CD11b and CD54. The nonparametric Mann Whitney U test was applied for data comparison, p value <0.05 being regarded as significant.The total number of cells were increased in asthma patients 4.81 ± 5.27x 106 /ml vs. healthy 2.8 ± 2.15 x 106/ml and it was statistically significant.Statistically significant increase in the percentage of eosinophils was observed in mild asthma subjects. No differences were found between the proportion in macrophages and lymphocytes. The macrophage phenothype in induced sputum differed in both groups. The expression of CD 11b was higher in asthma group and the difference was statistically significant.Theproportion of macrophages with the expression of CD 14, CD 71 and CD54 was comparable in both groups.
Macrophage phenotyping during glucocorticosteroid therapy is useful in the assessment of inflammatoryprocessin asthma subject.
Abstract
Macrophages represent the most predominant immune effector cells in the alveolar spaces and conducting
airways and are known to express activated phenotype. The study was aimed at assessing the differences in cellular profile and the expression of selected surface markers on sputum macrophages in asthma and healthy subjects.
17 healthy subjects (never smoked) and 10 mild asthma subjects treated with glucocorticosteroids were enrolled into the study. For macrophage phenotyping a immunocytochemistry method was used with commercially available
antibodies anti: CD14, CD71, CD11b and CD54. The nonparametric Mann Whitney U test was applied for data comparison, p value <0.05 being regarded as significant.The total number of cells were increased in asthma patients 4.81 ± 5.27x 106 /ml vs. healthy 2.8 ± 2.15 x 106/ml and it was statistically significant.Statistically significant increase in the percentage of eosinophils was observed in mild asthma subjects. No differences were found between the proportion in macrophages and lymphocytes. The macrophage phenothype in induced sputum differed in both groups. The expression of CD 11b was higher in asthma group and the difference was statistically significant.Theproportion of macrophages with the expression of CD 14, CD 71 and CD54 was comparable in both groups.
Macrophage phenotyping during glucocorticosteroid therapy is useful in the assessment of inflammatoryprocessin asthma subject.
Keywords
guinea pigs; dexamethasone; allergy asthma; phosphodiesterase4 inhibitor
Keywords
guinea pigs
dexamethasone
allergy asthma
phosphodiesterase4 inhibitor
Authors
Joanna Hermanowicz-Salamon
Joanna Domagała-Kulawik
Marta Maskey-Warzęchowska
Ryszarda Chazan