open access

Vol 74, No 1 (2006)
ORIGINAL PAPERS
Published online: 2008-02-18
Submitted: 2013-02-22
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The mycolic acids analysis with HPLC technique in drug susceptibility testing of Mycobacterium tuberculosis strains

Renata Walkiewicz, Hanna Grubek-Jaworska, Ryszarda Chazan
Pneumonol Alergol Pol 2006;74(1):89-94.

open access

Vol 74, No 1 (2006)
ORIGINAL PAPERS
Published online: 2008-02-18
Submitted: 2013-02-22

Abstract

The aim of this study was to evaluate the utility of the quantitative analysis of mycolic acids by HPLC technique in drug susceptibility testing of the M.tuberculosis isolates to the first-lineantituberculousdrugs:isoniazid and rifampicin. Drug susceptibility of the 30 clinical M.tbc isolates was examined by the mycolic acids analysis with HPLC technique and results were compared to the proportion method on solid L-J medium and liquid medium in MGIT system. In HPLC method drug susceptibility of M.tuberculosis 0.5, and TAMA indexes of susceptible strains were < 0.05. The average error of the TAMA analysis was &plusmn; 9.5% The quantitative analysis of mycolic acids by HPLC gives results compatible with standard proportion method and is a reliable method for determination of drug susceptibility of M.tuberculosis.

Abstract

The aim of this study was to evaluate the utility of the quantitative analysis of mycolic acids by HPLC technique in drug susceptibility testing of the M.tuberculosis isolates to the first-lineantituberculousdrugs:isoniazid and rifampicin. Drug susceptibility of the 30 clinical M.tbc isolates was examined by the mycolic acids analysis with HPLC technique and results were compared to the proportion method on solid L-J medium and liquid medium in MGIT system. In HPLC method drug susceptibility of M.tuberculosis 0.5, and TAMA indexes of susceptible strains were < 0.05. The average error of the TAMA analysis was &plusmn; 9.5% The quantitative analysis of mycolic acids by HPLC gives results compatible with standard proportion method and is a reliable method for determination of drug susceptibility of M.tuberculosis.
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Keywords

Mycolic acids; high pressure liquid chromatography HPLC; M.tuberculosis; drug resistance

About this article
Title

The mycolic acids analysis with HPLC technique in drug susceptibility testing of Mycobacterium tuberculosis strains

Journal

Advances in Respiratory Medicine

Issue

Vol 74, No 1 (2006)

Pages

89-94

Published online

2008-02-18

Bibliographic record

Pneumonol Alergol Pol 2006;74(1):89-94.

Keywords

Mycolic acids
high pressure liquid chromatography HPLC
M.tuberculosis
drug resistance

Authors

Renata Walkiewicz
Hanna Grubek-Jaworska
Ryszarda Chazan

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