Vol 74, No 1 (2006)
ORIGINAL PAPERS
Published online: 2008-02-18
Submitted: 2013-02-22
The mycolic acids analysis with HPLC technique in drug susceptibility testing of Mycobacterium tuberculosis strains
Renata Walkiewicz, Hanna Grubek-Jaworska, Ryszarda Chazan
Pneumonol Alergol Pol 2006;74(1):89-94.
Vol 74, No 1 (2006)
ORIGINAL PAPERS
Published online: 2008-02-18
Submitted: 2013-02-22
Abstract
The aim of this study was to evaluate the utility of the quantitative analysis of mycolic acids by HPLC technique in drug susceptibility testing of the M.tuberculosis isolates to the first-lineantituberculousdrugs:isoniazid and rifampicin. Drug susceptibility of the 30 clinical M.tbc isolates was examined by the mycolic acids analysis with HPLC technique and results were compared to the proportion method on solid L-J medium and liquid medium in MGIT system. In HPLC method drug susceptibility of M.tuberculosis strains was described by TAMA index defined as the ratio of the total area under mycolic acids peaks (TAMA) from cultures with drug to the TAMA of control. At critical concentrations of drugs, TAMA indexes of resistant strains were > 0.5, and TAMA indexes of susceptible strains were < 0.05. The average error of the TAMA analysis was ± 9.5% The quantitative analysis of mycolic acids by HPLC gives results compatible with standard proportion method and is a reliable method for determination of drug susceptibility of M.tuberculosis.
Abstract
The aim of this study was to evaluate the utility of the quantitative analysis of mycolic acids by HPLC technique in drug susceptibility testing of the M.tuberculosis isolates to the first-lineantituberculousdrugs:isoniazid and rifampicin. Drug susceptibility of the 30 clinical M.tbc isolates was examined by the mycolic acids analysis with HPLC technique and results were compared to the proportion method on solid L-J medium and liquid medium in MGIT system. In HPLC method drug susceptibility of M.tuberculosis strains was described by TAMA index defined as the ratio of the total area under mycolic acids peaks (TAMA) from cultures with drug to the TAMA of control. At critical concentrations of drugs, TAMA indexes of resistant strains were > 0.5, and TAMA indexes of susceptible strains were < 0.05. The average error of the TAMA analysis was ± 9.5% The quantitative analysis of mycolic acids by HPLC gives results compatible with standard proportion method and is a reliable method for determination of drug susceptibility of M.tuberculosis.
Keywords
Mycolic acids; high pressure liquid chromatography HPLC; M.tuberculosis; drug resistance
Title
The mycolic acids analysis with HPLC technique in drug susceptibility testing of Mycobacterium tuberculosis strains
Journal
Advances in Respiratory Medicine
Issue
Vol 74, No 1 (2006)
Pages
89-94
Published online
2008-02-18
Bibliographic record
Pneumonol Alergol Pol 2006;74(1):89-94.
Keywords
Mycolic acids
high pressure liquid chromatography HPLC
M.tuberculosis
drug resistance
Authors
Renata Walkiewicz
Hanna Grubek-Jaworska
Ryszarda Chazan
About this article