open access

Vol 74, No 1 (2006)
ORIGINAL PAPERS
Published online: 2008-02-18
Submitted: 2013-02-22
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Adenosine deaminase activity in tuberculous and malignant pleural effusions

Aleksandra Safianowska, Rafał Krenke, Barbara Dmowska-Sobstyl, Elżbieta Bogacka-Zatorska, Joanna Domagała-Kulawik, Ryszarda Chazan
Pneumonol Alergol Pol 2006;74(1):5-9.

open access

Vol 74, No 1 (2006)
ORIGINAL PAPERS
Published online: 2008-02-18
Submitted: 2013-02-22

Abstract

Measurement of pleural adenosine deaminase activity (ADA) is a useful diagnostic tool for tuberculous pleurisy, but false-positive findings from non-tuberculous effusions have been reported.
In order to improve diagnostic value of ADA it is recommended to estimate activity of both ADA1 and ADA2 izoenzymes or 2'-deoxyadenosine/adenosine activity ratio.
In order to evaluate ADA as a diagnostic parameter total ADA, with adenosine as a substrate, and 2'-deoxyadenosine/adenosine activity ratio were measured in tuberculous and malignant pleural effusions. Altogether, 26 pleural exudates (11 tuberculous and 15 malignant) were selected. ADA either with adenosine or 2'-deoxyadenosine was determined by colorimetric method of Giusti. Each pleural fluid sample was diluted prior to the assay(1:8)to avoid enzyme inhibition which was observed in nondiluted pleural effusions. The ADA level reached the diagnostic cut-off set for tuberculous effusions (40 U/L) in every 11 tuberculous exudates with the mean value of 85,3±47,1U/L; in 9 of these the 2'-deoxyadenosine/adenosine ratio was less than 0,45. In the malignant group of patients, no one ADA level exceed 40 U/L, being estimated at 10,6±7,7 U/L (p

Abstract

Measurement of pleural adenosine deaminase activity (ADA) is a useful diagnostic tool for tuberculous pleurisy, but false-positive findings from non-tuberculous effusions have been reported.
In order to improve diagnostic value of ADA it is recommended to estimate activity of both ADA1 and ADA2 izoenzymes or 2'-deoxyadenosine/adenosine activity ratio.
In order to evaluate ADA as a diagnostic parameter total ADA, with adenosine as a substrate, and 2'-deoxyadenosine/adenosine activity ratio were measured in tuberculous and malignant pleural effusions. Altogether, 26 pleural exudates (11 tuberculous and 15 malignant) were selected. ADA either with adenosine or 2'-deoxyadenosine was determined by colorimetric method of Giusti. Each pleural fluid sample was diluted prior to the assay(1:8)to avoid enzyme inhibition which was observed in nondiluted pleural effusions. The ADA level reached the diagnostic cut-off set for tuberculous effusions (40 U/L) in every 11 tuberculous exudates with the mean value of 85,3±47,1U/L; in 9 of these the 2'-deoxyadenosine/adenosine ratio was less than 0,45. In the malignant group of patients, no one ADA level exceed 40 U/L, being estimated at 10,6±7,7 U/L (p
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Keywords

adenosine deaminase activity; tuberculous pleurisy

About this article
Title

Adenosine deaminase activity in tuberculous and malignant pleural effusions

Journal

Advances in Respiratory Medicine

Issue

Vol 74, No 1 (2006)

Pages

5-9

Published online

2008-02-18

Bibliographic record

Pneumonol Alergol Pol 2006;74(1):5-9.

Keywords

adenosine deaminase activity
tuberculous pleurisy

Authors

Aleksandra Safianowska
Rafał Krenke
Barbara Dmowska-Sobstyl
Elżbieta Bogacka-Zatorska
Joanna Domagała-Kulawik
Ryszarda Chazan

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