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Vol 77, No 2 (2009)
Review paper
Published online: 2009-03-16
Submitted: 2013-02-22
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Flow cytometric basophils activation test as a method of allergy diagnosis

Olga Potapińska, Urszula Demkow, Maria Wąsik
Pneumonol Alergol Pol 2009;77(2):152-158.

open access

Vol 77, No 2 (2009)
REVIEWS
Published online: 2009-03-16
Submitted: 2013-02-22

Abstract

For several years the incidences of allergic diseases and anaphylactic reactions have been increasing dramatically. Classical method of allergy diagnosis - skin prick test in some situations can provoke life-threatening reactions. Detection of allergen-induced basophil activation by flow cytometry has been shown to be a useful tool for allergy diagnosis in those patients. CD 63 and CD203c have recently been demonstrated as a specific activation markers of basophils that are rapidly up-regulated after allergen challenge in sensitized patients. Although flow-cytometry methods are quite sophisticated and expensive, it could be a good alternative in patients at risk of severe anaphylactic reactions or with contradictory test results.

Abstract

For several years the incidences of allergic diseases and anaphylactic reactions have been increasing dramatically. Classical method of allergy diagnosis - skin prick test in some situations can provoke life-threatening reactions. Detection of allergen-induced basophil activation by flow cytometry has been shown to be a useful tool for allergy diagnosis in those patients. CD 63 and CD203c have recently been demonstrated as a specific activation markers of basophils that are rapidly up-regulated after allergen challenge in sensitized patients. Although flow-cytometry methods are quite sophisticated and expensive, it could be a good alternative in patients at risk of severe anaphylactic reactions or with contradictory test results.

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Keywords

flow cytometry; basophils; CD63; CD203c; allergy

About this article
Title

Flow cytometric basophils activation test as a method of allergy diagnosis

Journal

Advances in Respiratory Medicine

Issue

Vol 77, No 2 (2009)

Article type

Review paper

Pages

152-158

Published online

2009-03-16

Bibliographic record

Pneumonol Alergol Pol 2009;77(2):152-158.

Keywords

flow cytometry
basophils
CD63
CD203c
allergy

Authors

Olga Potapińska
Urszula Demkow
Maria Wąsik

References (16)
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