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Vol 77, No 2 (2009)
REVIEWS
Published online: 2009-03-16
Submitted: 2013-02-22
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Flow cytometric basophils activation test as a method of allergy diagnosis

Olga Potapińska, Urszula Demkow, Maria Wąsik
Pneumonol Alergol Pol 2009;77(2):152-158.

open access

Vol 77, No 2 (2009)
REVIEWS
Published online: 2009-03-16
Submitted: 2013-02-22

Abstract

For several years the incidences of allergic diseases and anaphylactic reactions have been increasing dramatically. Classical method of allergy diagnosis - skin prick test in some situations can provoke life-threatening reactions. Detection of allergen-induced basophil activation by flow cytometry has been shown to be a useful tool for allergy diagnosis in those patients. CD 63 and CD203c have recently been demonstrated as a specific activation markers of basophils that are rapidly up-regulated after allergen challenge in sensitized patients. Although flow-cytometry methods are quite sophisticated and expensive, it could be a good alternative in patients at risk of severe anaphylactic reactions or with contradictory test results.

Abstract

For several years the incidences of allergic diseases and anaphylactic reactions have been increasing dramatically. Classical method of allergy diagnosis - skin prick test in some situations can provoke life-threatening reactions. Detection of allergen-induced basophil activation by flow cytometry has been shown to be a useful tool for allergy diagnosis in those patients. CD 63 and CD203c have recently been demonstrated as a specific activation markers of basophils that are rapidly up-regulated after allergen challenge in sensitized patients. Although flow-cytometry methods are quite sophisticated and expensive, it could be a good alternative in patients at risk of severe anaphylactic reactions or with contradictory test results.

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Keywords

flow cytometry; basophils; CD63; CD203c; allergy

About this article
Title

Flow cytometric basophils activation test as a method of allergy diagnosis

Journal

Advances in Respiratory Medicine

Issue

Vol 77, No 2 (2009)

Pages

152-158

Published online

2009-03-16

Bibliographic record

Pneumonol Alergol Pol 2009;77(2):152-158.

Keywords

flow cytometry
basophils
CD63
CD203c
allergy

Authors

Olga Potapińska
Urszula Demkow
Maria Wąsik

References (16)
  1. Boumiza R, Monneret G, Forissier MF, et al. Marked improvement of the basophil activation test by detecting CD203c instead of CD63. Clin Exp Allergy. 2003; 33(2): 259–265.
    PubMed
  2. Valent P, Hauswirth AW, Natter S, et al. Assays for measuring in vitro basophil activation induced by recombinant allergens. Methods. 2004; 32(3): 265–270.
    CrossRefPubMed
  3. Sainte-Laudy J, Sabbah A, Drouet M, et al. Diagnosis of venom allergy by flow cytometry. Correlation with clinical history, skin tests, specific IgE, histamine and leukotriene C4 release. Clin Exp Allergy. 2000; 30(8): 1166–1171.
    PubMed
  4. Eberlein-König B, Schmidt-Leidescher C, Rakoski J, et al. In vitro basophil activation using CD63 expression in patients with bee and wasp venom allergy. J Investig Allergol Clin Immunol. 2006; 16(1): 5–10.
    PubMed
  5. Crockard AD, Ennis M. Laboratory-based allergy diagnosis: should we go with the flow? Clin Exp Allergy. 2001; 31(7): 975–977.
    PubMed
  6. Boumiza R, Debard AL, Monneret G. The basophil activation test by flow cytometry: recent developments in clinical studies, standardization and emerging perspectives. Clin Mol Allergy. 2005; 3: 9.
    CrossRefPubMed
  7. Triggiani M, Marone G. Basophil's secrets revealed by flow cytometry. Allergy. 2006; 61(9): 1025–1027.
    CrossRefPubMed
  8. Michova A, Abugalia M, Ivanova Ts, et al. Comparision of two-flow cytometry methods for basophil degranulation in patients sensitized to grass pollen. Allergy. 2006; 61(9): 1078–1083.
    CrossRefPubMed
  9. Ebo DG, Sainte-Laudy J, Bridts CH, et al. Flow-assisted allergy diagnosis: current applications and future perspectives. Allergy. 2006; 61(9): 1028–1039.
    CrossRefPubMed
  10. Lambert C, Guilloux L, Dzviga C, et al. Flow cytometry versus histamine release analysis of in vitro basophil degranulation in allergy to Hymenoptera venom. Cytometry B Clin Cytom. 2003; 52(1): 13–19.
    CrossRefPubMed
  11. Dubois AEJ, van der Heide S. Basophil-activation tests in Hymenoptera allergy. Curr Opin Allergy Clin Immunol. 2007; 7(4): 346–349.
    CrossRefPubMed
  12. Eberlein-König B, Rakoski J, Behrendt H, et al. Use of CD63 expression as marker of in vitro basophil activation in identifying the culprit in insect venom allergy. J Investig Allergol Clin Immunol. 2004; 14(1): 10–16.
    PubMed
  13. Sudheer PS, Hall JE, Read GF, et al. Flow cytometric investigation of peri-anaesthetic anaphylaxis using CD63 and CD203c. Anaesthesia. 2005; 60(3): 251–256.
    CrossRefPubMed
  14. Eberlein-König B, Varga R, Mempel M, et al. Comparison of basophil activation tests using CD63 or CD203c expression in patients with insect venom allergy. Allergy. 2006; 61(9): 1084–1085.
    CrossRefPubMed
  15. Hemery ML, Arnoux B, Dhivert-Donnadieu H, et al. Confirmation of the diagnosis of natural rubber latex allergy by the Basotest method. Int Arch Allergy Immunol. 2005; 136(1): 53–57.
    CrossRefPubMed
  16. Ocmant A, Peignois Y, Mulier S, et al. Flow cytometry for basophil activation markers: the measurement of CD203c up-regulation is as reliable as CD63 expression in the diagnosis of cat allergy. J Immunol Methods. 2007; 320(1-2): 40–48.
    CrossRefPubMed

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