open access

Vol 77, No 5 (2009)
ORIGINAL PAPERS
Published online: 2009-08-12
Submitted: 2013-02-22
Get Citation

Comparison of Th1 and Th2 response in the blood of tuberculous patients and healthy contacts

Jolanta Winek, Urszula Demkow, Ewa Rowińska-Zakrzewska, Małgorzata Szołkowska, Małgorzata Filewska, Jacek Jagodziński, Kazimierz Roszkowski-Śliż
Pneumonol Alergol Pol 2009;77(5):446-452.

open access

Vol 77, No 5 (2009)
ORIGINAL PAPERS
Published online: 2009-08-12
Submitted: 2013-02-22

Abstract


Introduction: Th1 response is known to play a dominant role in the resistance to tuberculosis. Nevertheless, IFN gamma levels are frequently increased in tuberculous patients, especially at the site of the disease. It is also possible that the shift toward Th2 response is responsible for the loss of resistance. The aim of this study was to compare the Th1 function of peripheral blood cells and the levels of antimycobacterial antibodies in the serum of culture positive tuberculosis patients and healthy tuberculosis (Tb) contacts. The correlation between the levels of antimycobacterial antibodies and Th1 function of blood cells was also evaluated.
Material and methods: The material consisted of 51 tuberculous patients and 20 healthy persons, close contacts of tuberculosis patients. The ability of peripheral blood cells to secrete IFN gamma and IL-2 was estimated in whole blood cultures with PHA, PWM and tuberculin. The levels of IFN gamma and IL-2 in the supernatants of cultures was estimated via a commercial ELISA test. The levels of antimycobacterial antibodies was measured with commercial immunoenzymatic kits detecting IgG antibodies against 38 kDa+16 kDa and IgG, IgA and IgM antibodies to 38 kDa + lipoarabinomannan (LAM).
Results: No difference was found in the secretion of IFN gamma and IL-2 after stimulation with PHA and PWM between the patients and contacts. The secretion of IFN gamma after stimulation with tuberculin was even greater in tuberculous patients than in contacts. The levels of IgG and IgA (38 kDa+LAM) were higher in tuberculous patients than in contacts. There was a negative correlation between the level of IgG anti 38 kDa+LAM and the ability of peripheral blood cells to secrete IFN gamma after non-specific stimulation in patients with tuberculosis.
Conclusions: Our study confirms the hypothesis that it is not the diminished production of Th1 cytokines, but rather the parallel overproduction of Th2 cytokines, which are essential in the development of tuberculosis.

Abstract


Introduction: Th1 response is known to play a dominant role in the resistance to tuberculosis. Nevertheless, IFN gamma levels are frequently increased in tuberculous patients, especially at the site of the disease. It is also possible that the shift toward Th2 response is responsible for the loss of resistance. The aim of this study was to compare the Th1 function of peripheral blood cells and the levels of antimycobacterial antibodies in the serum of culture positive tuberculosis patients and healthy tuberculosis (Tb) contacts. The correlation between the levels of antimycobacterial antibodies and Th1 function of blood cells was also evaluated.
Material and methods: The material consisted of 51 tuberculous patients and 20 healthy persons, close contacts of tuberculosis patients. The ability of peripheral blood cells to secrete IFN gamma and IL-2 was estimated in whole blood cultures with PHA, PWM and tuberculin. The levels of IFN gamma and IL-2 in the supernatants of cultures was estimated via a commercial ELISA test. The levels of antimycobacterial antibodies was measured with commercial immunoenzymatic kits detecting IgG antibodies against 38 kDa+16 kDa and IgG, IgA and IgM antibodies to 38 kDa + lipoarabinomannan (LAM).
Results: No difference was found in the secretion of IFN gamma and IL-2 after stimulation with PHA and PWM between the patients and contacts. The secretion of IFN gamma after stimulation with tuberculin was even greater in tuberculous patients than in contacts. The levels of IgG and IgA (38 kDa+LAM) were higher in tuberculous patients than in contacts. There was a negative correlation between the level of IgG anti 38 kDa+LAM and the ability of peripheral blood cells to secrete IFN gamma after non-specific stimulation in patients with tuberculosis.
Conclusions: Our study confirms the hypothesis that it is not the diminished production of Th1 cytokines, but rather the parallel overproduction of Th2 cytokines, which are essential in the development of tuberculosis.
Get Citation

Keywords

Th-1; Th2 response in tuberculosis; IFN gamma; IL-2; antimycobacterial antibodies

About this article
Title

Comparison of Th1 and Th2 response in the blood of tuberculous patients and healthy contacts

Journal

Advances in Respiratory Medicine

Issue

Vol 77, No 5 (2009)

Pages

446-452

Published online

2009-08-12

Bibliographic record

Pneumonol Alergol Pol 2009;77(5):446-452.

Keywords

Th-1
Th2 response in tuberculosis
IFN gamma
IL-2
antimycobacterial antibodies

Authors

Jolanta Winek
Urszula Demkow
Ewa Rowińska-Zakrzewska
Małgorzata Szołkowska
Małgorzata Filewska
Jacek Jagodziński
Kazimierz Roszkowski-Śliż

Important: This website uses cookies.tanya dokter More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

Czasopismo Pneumonologia i Alergologia Polska dostęne jest również w Ikamed - księgarnia medyczna

Wydawcą serwisu jest "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail: viamedica@viamedica.pl