open access

Vol 77, No 6 (2009)
ORIGINAL PAPERS
Published online: 2009-11-30
Submitted: 2013-02-22
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Diagnostic utility of the molecular assay GenoType MTBC (HAIN Lifesciences, Germany) for identification of tuberculous mycobacteria

Aleksandra Safianowska, Renata Walkiewicz, Patrycja Nejman-Gryz, Ryszarda Chazan, Hanna Grubek-Jaworska
Pneumonol Alergol Pol 2009;77(6):517-520.

open access

Vol 77, No 6 (2009)
ORIGINAL PAPERS
Published online: 2009-11-30
Submitted: 2013-02-22

Abstract


Introduction: The GenoType system (HAIN Lifescience, Germany) offers new perspectives of detecting the tuberculous and non-tuberculous mycobacteria at the molecular level. The system compromises five independent tests that could be performed either on direct specimens or isolated strains, to identify the strains and test the resistance against rifampin and isoniazid. Up to now, non GenoType test was applied in Poland. The aim of the study was an evaluation the accuracy of GenoType MTBC test in speciation of the clinical isolates, previously classified as M. tuberculosis complex by HPLC analyze of mycolic acids.
Material and methods: 161 clinical isolates, derived from the TB patients hospitalized in the Warsaw Medical University Hospital between 1999 and 2007 were assayed.
Results: On the basis of the hybridization patterns, all 161 studied strains were identified as M. tuberculosis/M. canettii.
Conclusions:
1. The GenoType MTBC test (HAIN Lifescience, Germany) precisely recognizes M. tuberculosis complex. The 100% accordance in speciation of M. tuberculosis by the GenoType MTBC test as compared to HPLC method was demonstrated. The GenoType MTBC test can replace HPLC in detection of tuberculous mycobacteria in clinical isolates.
2. As the GenoType MTBC test performs well, the other tests of GenoType system may be considered to be verified in diagnostic procedure of mycobacterial infection.

Abstract


Introduction: The GenoType system (HAIN Lifescience, Germany) offers new perspectives of detecting the tuberculous and non-tuberculous mycobacteria at the molecular level. The system compromises five independent tests that could be performed either on direct specimens or isolated strains, to identify the strains and test the resistance against rifampin and isoniazid. Up to now, non GenoType test was applied in Poland. The aim of the study was an evaluation the accuracy of GenoType MTBC test in speciation of the clinical isolates, previously classified as M. tuberculosis complex by HPLC analyze of mycolic acids.
Material and methods: 161 clinical isolates, derived from the TB patients hospitalized in the Warsaw Medical University Hospital between 1999 and 2007 were assayed.
Results: On the basis of the hybridization patterns, all 161 studied strains were identified as M. tuberculosis/M. canettii.
Conclusions:
1. The GenoType MTBC test (HAIN Lifescience, Germany) precisely recognizes M. tuberculosis complex. The 100% accordance in speciation of M. tuberculosis by the GenoType MTBC test as compared to HPLC method was demonstrated. The GenoType MTBC test can replace HPLC in detection of tuberculous mycobacteria in clinical isolates.
2. As the GenoType MTBC test performs well, the other tests of GenoType system may be considered to be verified in diagnostic procedure of mycobacterial infection.
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Keywords

hybridization; multiplex PCR; Mycobacterium tuberculosis complex

About this article
Title

Diagnostic utility of the molecular assay GenoType MTBC (HAIN Lifesciences, Germany) for identification of tuberculous mycobacteria

Journal

Advances in Respiratory Medicine

Issue

Vol 77, No 6 (2009)

Pages

517-520

Published online

2009-11-30

Bibliographic record

Pneumonol Alergol Pol 2009;77(6):517-520.

Keywords

hybridization
multiplex PCR
Mycobacterium tuberculosis complex

Authors

Aleksandra Safianowska
Renata Walkiewicz
Patrycja Nejman-Gryz
Ryszarda Chazan
Hanna Grubek-Jaworska

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