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Vol 78, No 5 (2010)
Research paper
Published online: 2010-08-10
Submitted: 2013-02-22
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The comparison between two methods for typing of nontuberculous mycobacteria: high pressure liquid chromatography and molecular assay GenoType Mycobacterium CM/AS

Aleksandra Safianowska, Renata Walkiewicz, Patrycja Nejman-Gryz, Ryszarda Chazan, Hanna Grubek-Jaworska
Pneumonol Alergol Pol 2010;78(5):363-368.

open access

Vol 78, No 5 (2010)
ORIGINAL PAPERS
Published online: 2010-08-10
Submitted: 2013-02-22

Abstract


Introduction: The GenoType Mycobacterium CM and the GenoType Mycobacterium AS (HAIN Lifescience, Germany) were evaluated for the ability to differentiate mycobacterial species of clinical isolates. Serial use of the both assays is aimed to identify 38 different molecular patterns, of which 24 patterns can be assigned to single species, 10 patterns are allocated to two or more Mycobacterium species, and 4 patterns correspond to Mycobacterium species and gram-positive bacteria with a high G + C content. The analysis of mycolic acids by high pressure liquid chromatography (HPLC) was the reference method.
Material and methods: A set of 127 nontuberculous mycobacterial isolates on Loewenstein-Jensen slants, derived from different patients between 1999 and 2007, was analyzed. The strains were primary classified by HPLC following the diagnostic procedure, and retyped by GenoType Mycobacterium CM/AS.
Results: In total, results obtained by both methods were interpretable for 113 strains. Concordant results were obtained for 105 (93%) mycobacterial strains. One out of 8 inconcordant classified strains, which was classified as by HPLC, displayed a pattern of M. tuberculosis complex by a molecular method. Eleven clinical strains were differentiated only by one of used methods, either by HPLC (6 strains) or by GenoType CM/ AS (5 strains). Three strains were not classified at all.
Conclusions: Our results show that the GenoType Mycobacterium CM/AS system represents a useful tool to identify mycobacterial clinical isolates. The molecular system is as rapid and reliable as the HPLC, but much easier to perform and more friendly for the environment.
Pneumonol. Alergol. Pol. 2010; 78, 5: 363-368

Abstract


Introduction: The GenoType Mycobacterium CM and the GenoType Mycobacterium AS (HAIN Lifescience, Germany) were evaluated for the ability to differentiate mycobacterial species of clinical isolates. Serial use of the both assays is aimed to identify 38 different molecular patterns, of which 24 patterns can be assigned to single species, 10 patterns are allocated to two or more Mycobacterium species, and 4 patterns correspond to Mycobacterium species and gram-positive bacteria with a high G + C content. The analysis of mycolic acids by high pressure liquid chromatography (HPLC) was the reference method.
Material and methods: A set of 127 nontuberculous mycobacterial isolates on Loewenstein-Jensen slants, derived from different patients between 1999 and 2007, was analyzed. The strains were primary classified by HPLC following the diagnostic procedure, and retyped by GenoType Mycobacterium CM/AS.
Results: In total, results obtained by both methods were interpretable for 113 strains. Concordant results were obtained for 105 (93%) mycobacterial strains. One out of 8 inconcordant classified strains, which was classified as by HPLC, displayed a pattern of M. tuberculosis complex by a molecular method. Eleven clinical strains were differentiated only by one of used methods, either by HPLC (6 strains) or by GenoType CM/ AS (5 strains). Three strains were not classified at all.
Conclusions: Our results show that the GenoType Mycobacterium CM/AS system represents a useful tool to identify mycobacterial clinical isolates. The molecular system is as rapid and reliable as the HPLC, but much easier to perform and more friendly for the environment.
Pneumonol. Alergol. Pol. 2010; 78, 5: 363-368

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Keywords

GenoType Mycobacterium CM/AS; high pressure liquid chromatography (HPLC); nontuberculous mykobacteria (NTM)

About this article
Title

The comparison between two methods for typing of nontuberculous mycobacteria: high pressure liquid chromatography and molecular assay GenoType Mycobacterium CM/AS

Journal

Advances in Respiratory Medicine

Issue

Vol 78, No 5 (2010)

Article type

Research paper

Pages

363-368

Published online

2010-08-10

Bibliographic record

Pneumonol Alergol Pol 2010;78(5):363-368.

Keywords

GenoType Mycobacterium CM/AS
high pressure liquid chromatography (HPLC)
nontuberculous mykobacteria (NTM)

Authors

Aleksandra Safianowska
Renata Walkiewicz
Patrycja Nejman-Gryz
Ryszarda Chazan
Hanna Grubek-Jaworska

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