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Vol 78, No 5 (2010)
Research paper
Submitted: 2013-02-22
Published online: 2010-08-10
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The comparison between two methods for typing of nontuberculous mycobacteria: high pressure liquid chromatography and molecular assay GenoType Mycobacterium CM/AS

Aleksandra Safianowska, Renata Walkiewicz, Patrycja Nejman-Gryz, Ryszarda Chazan, Hanna Grubek-Jaworska
Pneumonol Alergol Pol 2010;78(5):363-368.

open access

Vol 78, No 5 (2010)
ORIGINAL PAPERS
Submitted: 2013-02-22
Published online: 2010-08-10

Abstract


Introduction: The GenoType Mycobacterium CM and the GenoType Mycobacterium AS (HAIN Lifescience, Germany) were evaluated for the ability to differentiate mycobacterial species of clinical isolates. Serial use of the both assays is aimed to identify 38 different molecular patterns, of which 24 patterns can be assigned to single species, 10 patterns are allocated to two or more Mycobacterium species, and 4 patterns correspond to Mycobacterium species and gram-positive bacteria with a high G + C content. The analysis of mycolic acids by high pressure liquid chromatography (HPLC) was the reference method.
Material and methods: A set of 127 nontuberculous mycobacterial isolates on Loewenstein-Jensen slants, derived from different patients between 1999 and 2007, was analyzed. The strains were primary classified by HPLC following the diagnostic procedure, and retyped by GenoType Mycobacterium CM/AS.
Results: In total, results obtained by both methods were interpretable for 113 strains. Concordant results were obtained for 105 (93%) mycobacterial strains. One out of 8 inconcordant classified strains, which was classified as by HPLC, displayed a pattern of M. tuberculosis complex by a molecular method. Eleven clinical strains were differentiated only by one of used methods, either by HPLC (6 strains) or by GenoType CM/ AS (5 strains). Three strains were not classified at all.
Conclusions: Our results show that the GenoType Mycobacterium CM/AS system represents a useful tool to identify mycobacterial clinical isolates. The molecular system is as rapid and reliable as the HPLC, but much easier to perform and more friendly for the environment.
Pneumonol. Alergol. Pol. 2010; 78, 5: 363-368

Abstract


Introduction: The GenoType Mycobacterium CM and the GenoType Mycobacterium AS (HAIN Lifescience, Germany) were evaluated for the ability to differentiate mycobacterial species of clinical isolates. Serial use of the both assays is aimed to identify 38 different molecular patterns, of which 24 patterns can be assigned to single species, 10 patterns are allocated to two or more Mycobacterium species, and 4 patterns correspond to Mycobacterium species and gram-positive bacteria with a high G + C content. The analysis of mycolic acids by high pressure liquid chromatography (HPLC) was the reference method.
Material and methods: A set of 127 nontuberculous mycobacterial isolates on Loewenstein-Jensen slants, derived from different patients between 1999 and 2007, was analyzed. The strains were primary classified by HPLC following the diagnostic procedure, and retyped by GenoType Mycobacterium CM/AS.
Results: In total, results obtained by both methods were interpretable for 113 strains. Concordant results were obtained for 105 (93%) mycobacterial strains. One out of 8 inconcordant classified strains, which was classified as by HPLC, displayed a pattern of M. tuberculosis complex by a molecular method. Eleven clinical strains were differentiated only by one of used methods, either by HPLC (6 strains) or by GenoType CM/ AS (5 strains). Three strains were not classified at all.
Conclusions: Our results show that the GenoType Mycobacterium CM/AS system represents a useful tool to identify mycobacterial clinical isolates. The molecular system is as rapid and reliable as the HPLC, but much easier to perform and more friendly for the environment.
Pneumonol. Alergol. Pol. 2010; 78, 5: 363-368

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Keywords

GenoType Mycobacterium CM/AS; high pressure liquid chromatography (HPLC); nontuberculous mykobacteria (NTM)

About this article
Title

The comparison between two methods for typing of nontuberculous mycobacteria: high pressure liquid chromatography and molecular assay GenoType Mycobacterium CM/AS

Journal

Advances in Respiratory Medicine

Issue

Vol 78, No 5 (2010)

Article type

Research paper

Pages

363-368

Published online

2010-08-10

Bibliographic record

Pneumonol Alergol Pol 2010;78(5):363-368.

Keywords

GenoType Mycobacterium CM/AS
high pressure liquid chromatography (HPLC)
nontuberculous mykobacteria (NTM)

Authors

Aleksandra Safianowska
Renata Walkiewicz
Patrycja Nejman-Gryz
Ryszarda Chazan
Hanna Grubek-Jaworska

References (24)
  1. Euzéby J.P. List of Prokaryotic names with Standing in Nomenclature — Genus Mycobacterium. http://www.bacterio.cict.fr/m/mycobacterium.html (08.07.2010).
  2. Falkinham JO. Epidemiology of infection by nontuberculous mycobacteria. Clin Microbiol Rev. 1996; 9: 177–215.
  3. Lavania M, Katoch K, Parashar D, et al. Infections due to non-tuberculous mycobacteria (NTM). Indian J Med Res. 2004; 120(4): 290–304.
    PubMed
  4. Griffith D, Aksamit T, Brown-Elliott B, et al. An Official ATS/IDSA Statement: Diagnosis, Treatment, and Prevention of Nontuberculous Mycobacterial Diseases. American Journal of Respiratory and Critical Care Medicine. 2007; 175(4): 367–416.
    CrossRef
  5. Management of opportunist mycobacterial infections: Joint Tuberculosis Committee Guidelines 1999. Subcommittee of the Joint Tuberculosis Committee of the British Thoracic Society. Thorax. 2000; 55(3): 210–218.
    PubMed
  6. Shitrit D, Priess R, Peled N, et al. Differentiation of Mycobacterium kansasii infection from Mycobacterium tuberculosis infection: comparison of clinical features, radiological appearance, and outcome. Eur J Clin Microbiol Infect Dis. 2007; 26(10): 679–684.
    CrossRefPubMed
  7. d'Arminio Monforte A, Vago L, Gori A, et al. Clinical diagnosis of mycobacterial diseases versus autopsy findings in 350 patients with AIDS. Eur J Clin Microbiol Infect Dis. 1996; 15(6): 453–458.
    PubMed
  8. Grubek-Jaworska H, Walkiewicz R, Safianowska A, et al. Nontuberculous mycobacterial infections among patients suspected of pulmonary tuberculosis. Eur J Clin Microbiol Infect Dis. 2009; 28(7): 739–744.
    CrossRefPubMed
  9. Debrunner M, Salfinger M, Brändli O, et al. Epidemiology and clinical significance of nontuberculous mycobacteria in patients negative for human immunodeficiency virus in Switzerland. Clin Infect Dis. 1992; 15(2): 330–345.
    PubMed
  10. Henry MT, Inamdar L, O'Riordain D, et al. Nontuberculous mycobacteria in non-HIV patients: epidemiology, treatment and response. Eur Respir J. 2004; 23(5): 741–746.
    PubMed
  11. Marras TK, Daley CL. Epidemiology of human pulmonary infection with nontuberculous mycobacteria. Clin Chest Med. 2002; 23(3): 553–567.
    PubMed
  12. Adle-Biassette H, Huerre M, Breton G, et al. [Non tuberculous mycobacterial diseases]. Ann Pathol. 2003; 23(3): 216–235.
    PubMed
  13. Butler WR, Guthertz LS. Mycolic acid analysis by high-performance liquid chromatography for identification of Mycobacterium species. Clin Microbiol Rev. 2001; 14(4): 704–726.
    CrossRefPubMed
  14. Safianowska A, Walkiewicz R, Nejman-Gryz P, et al. [Mycolic acids analysis from various species mycobacterium by high pressure liquid chromatography (HPLC)]. Pneumonol Alergol Pol. 2002; 70(3-4): 130–138.
    PubMed
  15. Walkiewicz R, Safianowska A, Grubek-Jaworska H, et al. Zastosowanie analizy kwasów mikolowych w diagnostyce gruźlicy i mikobakterioz — 3 lata doświadczeń. Pneumonol Alergol Pol. 2002; 70: 444–449.
  16. Daley P, Petrich A, May K, et al. Comparison of in-house and commercial 16S rRNA sequencing with high-performance liquid chromatography and genotype AS and CM for identification of nontuberculous mycobacteria. Diagn Microbiol Infect Dis. 2008; 61(3): 284–293.
    CrossRefPubMed
  17. Przondo-Mordarska A. Podstawowe procedury laboratoryjne w bakteriologii klinicznej. Wyd. Lek. PZWL, Warszawa 2005: 86–87.
  18. Standarized Method for HPLC Identification of Mycobacteria. U.S. Departament of Heath and Human Services, CDC. http://www.cdc.gov/ncidod/publications/hplc.pdf (1996).
  19. Safianowska A, Walkiewicz R, Nejman-Gryz P, et al. Przydatność diagnostyczna molekularnego testu GenoType MTBC (HAIN Lifescience, Niemcy) w identyfikacji prątków gruźlicy. Pneumonol Alergol Pol. 2009; 77: 517–520.
  20. Butler WR, Ahearn DG, Kilburn JO. High-performance liquid chromatography of mycolic acids as a tool in the identification of Corynebacterium, Nocardia, Rhodococcus, and Mycobacterium species. J Clin Microbiol. 1986; 23(1): 182–185.
    PubMed
  21. Astola J, Muñoz M, Sempere M, et al. The HPLC-double-cluster pattern of some Mycobacterium gordonae strains is due to their dicarboxy-mycolate content. Microbiology. 2002; 148(Pt 10): 3119–3127.
    CrossRefPubMed
  22. Gitti Z, Neonakis I, Fanti G, et al. Use of the GenoType Mycobacterium CM and AS assays to analyze 76 nontuberculous mycobacterial isolates from Greece. J Clin Microbiol. 2006; 44(6): 2244–2246.
    CrossRefPubMed
  23. Richter E, Rüsch-Gerdes S, Hillemann D. Evaluation of the GenoType Mycobacterium Assay for identification of mycobacterial species from cultures. J Clin Microbiol. 2006; 44(5): 1769–1775.
    CrossRefPubMed
  24. Sarkola A, Mäkinen J, Marjamäki M, et al. Prospective evaluation of the GenoType assay for routine identification of mycobacteria. Eur J Clin Microbiol Infect Dis. 2004; 23(8): 642–645.
    CrossRefPubMed

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