open access

Vol 79, No 4 (2011)
ORIGINAL PAPERS
Published online: 2011-06-15
Submitted: 2013-02-22
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Interferon-gamma assays T-SPOT.TB for the diagnosis of latent tuberculosis infection

Dagmara Borkowska, Zofia Zwolska, Dorota Michałowska-Mitczuk, Maria Korzeniewska-- Koseła, Anna Zabost, Agnieszka Napiórkowska, Monika Kozińska, Sylwia Brzezińska, Ewa Augustynowicz-Kopeć
Pneumonol Alergol Pol 2011;79(4):264-271.

open access

Vol 79, No 4 (2011)
ORIGINAL PAPERS
Published online: 2011-06-15
Submitted: 2013-02-22

Abstract

Introduction: The diagnosis of latent tuberculosis infection (LTBI) is currently based on the century-old tuberculin skin test (TST). However a positive reaction can result from infection by Mycobacterium tuberculosis, BCG vaccination or cross-reaction with nontuberculous mycobacteria. T-SPOT.TB assay is a new test to diagnose tuberculosis infection by measuring in vitro T-cell interferon gamma release in response to two Mycobacterium tuberculosis-specific antigens: ESAT-6 and CFP 10.
Material and methods: T-SPOT.TB assay has been performed on whole blood samples (n = 137) from March to September 2010. A tuberculin skin test result was available for 96 of participants. A positive TST result was considered if the induration was 10 mm or more.
Results: Of the 137 patients tested, T-SPOT.TB assay results were positive in 37 (27%), negative in 98 (71.5%) and indeterminate in only 2 (1.5%) persons. We analyzed T-SPOT.TB and TST results in the 96 patients for whom both test were available. Concordance between T-SPOT.TB and TST results (10 mm skin reaction interpreted as positive) was 79%. Fifteen (15.6%) patients had a positive TST result and a negative T-SPOT.TB and 5 (5.2%) patients had a negative TST result and a positive T-SPOT.TB. We observed good correlation between positive T-SPOT.TB results and the size of induration ≥ 15 mm in TST results.
Conclusions: T-SPOT.TB offers a more accurate approach than TST for identification tuberculosis infection. The study shows that the test T-SPOT.TB is a good diagnostic tool in identifying persons with tuberculosis infection. For full confirmation of this assessment, it is necessary to examine more cases.
Pneumonol. Alergol. Pol. 2011; 79, 4: 264–271

Abstract

Introduction: The diagnosis of latent tuberculosis infection (LTBI) is currently based on the century-old tuberculin skin test (TST). However a positive reaction can result from infection by Mycobacterium tuberculosis, BCG vaccination or cross-reaction with nontuberculous mycobacteria. T-SPOT.TB assay is a new test to diagnose tuberculosis infection by measuring in vitro T-cell interferon gamma release in response to two Mycobacterium tuberculosis-specific antigens: ESAT-6 and CFP 10.
Material and methods: T-SPOT.TB assay has been performed on whole blood samples (n = 137) from March to September 2010. A tuberculin skin test result was available for 96 of participants. A positive TST result was considered if the induration was 10 mm or more.
Results: Of the 137 patients tested, T-SPOT.TB assay results were positive in 37 (27%), negative in 98 (71.5%) and indeterminate in only 2 (1.5%) persons. We analyzed T-SPOT.TB and TST results in the 96 patients for whom both test were available. Concordance between T-SPOT.TB and TST results (10 mm skin reaction interpreted as positive) was 79%. Fifteen (15.6%) patients had a positive TST result and a negative T-SPOT.TB and 5 (5.2%) patients had a negative TST result and a positive T-SPOT.TB. We observed good correlation between positive T-SPOT.TB results and the size of induration ≥ 15 mm in TST results.
Conclusions: T-SPOT.TB offers a more accurate approach than TST for identification tuberculosis infection. The study shows that the test T-SPOT.TB is a good diagnostic tool in identifying persons with tuberculosis infection. For full confirmation of this assessment, it is necessary to examine more cases.
Pneumonol. Alergol. Pol. 2011; 79, 4: 264–271
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Keywords

latent tuberculosis infection; IGRA tests; tuberculin skin test; interferon g; antigens ESAT-6 and CFP 10

About this article
Title

Interferon-gamma assays T-SPOT.TB for the diagnosis of latent tuberculosis infection

Journal

Advances in Respiratory Medicine

Issue

Vol 79, No 4 (2011)

Pages

264-271

Published online

2011-06-15

Bibliographic record

Pneumonol Alergol Pol 2011;79(4):264-271.

Keywords

latent tuberculosis infection
IGRA tests
tuberculin skin test
interferon g
antigens ESAT-6 and CFP 10

Authors

Dagmara Borkowska
Zofia Zwolska
Dorota Michałowska-Mitczuk
Maria Korzeniewska-- Koseła
Anna Zabost
Agnieszka Napiórkowska
Monika Kozińska
Sylwia Brzezińska
Ewa Augustynowicz-Kopeć

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