open access

Vol 80, No 4 (2012)
ORIGINAL PAPERS
Published online: 2012-06-19
Submitted: 2013-02-22
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The effect of intermittent hypoxic training on lung and heart tissues of healthy rats

Katerina Rozova, Iryna Mankovska
Pneumonol Alergol Pol 2012;80(4):296-300.

open access

Vol 80, No 4 (2012)
ORIGINAL PAPERS
Published online: 2012-06-19
Submitted: 2013-02-22

Abstract

Introduction: Recently, particular attention has been focused on the problem of the beneficial influence of intermittent hypoxia (IH) on the human organism. However, knowledge regarding the negative effects of intermittent hypoxic training (IHT) on cellular adaptive mechanisms remains limited. The aim of the present study was to investigate: 1) lung and heart ultrastructural changes under IHT; and 2) the adequateness of morphological and morphometric methods to determine the constructive and destructive displays of hypoxia.
Material and methods: Adult male Wistar rats underwent IHT every day for 7–28 days. Lung and heart tissues were assessed by morphological and cellular morphometric methods.
Results: We observed evident ultra structural changes of the lung air-blood barrier (LABB) by the 7–10th day of training. Structural damage of LABB was most considerable after 2 weeks of IHT exposure, its ultrastructure partially normalized by the end of the IHT 4-weeks course: there was diminishing of LABB hydration and disappearance of areas of its destruction. The structural changes in the heart blood-tissue barrier (HBTB) were considerably less marked compared with those in LABB during the 1st and 2nd weeks of training. Heart tissue structural changes increased by the end of the fourth week of IHT. Both tissue cells revealed no significant necrotic damage of mitochondria after IHT, while changes relating to the energy-directed restructuring of mitochondria were observed. We hypothesized that acute moderate hypoxia promotes a specific type of mitosis in lung and heart tissues.
Conclusions: Ultrastructural changes in the rat lung and heart tissues depend on IHT duration. The phenomenon of “micromitochondria within mitochondria” is an additional adaptive mechanism for IH exposure.

Abstract

Introduction: Recently, particular attention has been focused on the problem of the beneficial influence of intermittent hypoxia (IH) on the human organism. However, knowledge regarding the negative effects of intermittent hypoxic training (IHT) on cellular adaptive mechanisms remains limited. The aim of the present study was to investigate: 1) lung and heart ultrastructural changes under IHT; and 2) the adequateness of morphological and morphometric methods to determine the constructive and destructive displays of hypoxia.
Material and methods: Adult male Wistar rats underwent IHT every day for 7–28 days. Lung and heart tissues were assessed by morphological and cellular morphometric methods.
Results: We observed evident ultra structural changes of the lung air-blood barrier (LABB) by the 7–10th day of training. Structural damage of LABB was most considerable after 2 weeks of IHT exposure, its ultrastructure partially normalized by the end of the IHT 4-weeks course: there was diminishing of LABB hydration and disappearance of areas of its destruction. The structural changes in the heart blood-tissue barrier (HBTB) were considerably less marked compared with those in LABB during the 1st and 2nd weeks of training. Heart tissue structural changes increased by the end of the fourth week of IHT. Both tissue cells revealed no significant necrotic damage of mitochondria after IHT, while changes relating to the energy-directed restructuring of mitochondria were observed. We hypothesized that acute moderate hypoxia promotes a specific type of mitosis in lung and heart tissues.
Conclusions: Ultrastructural changes in the rat lung and heart tissues depend on IHT duration. The phenomenon of “micromitochondria within mitochondria” is an additional adaptive mechanism for IH exposure.
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Keywords

intermittent hypoxic training; ultra-structural lung and heart tissues changes; biological barriers thickness; mitochondria; micro-mitochondria

About this article
Title

The effect of intermittent hypoxic training on lung and heart tissues of healthy rats

Journal

Advances in Respiratory Medicine

Issue

Vol 80, No 4 (2012)

Pages

296-300

Published online

2012-06-19

Bibliographic record

Pneumonol Alergol Pol 2012;80(4):296-300.

Keywords

intermittent hypoxic training
ultra-structural lung and heart tissues changes
biological barriers thickness
mitochondria
micro-mitochondria

Authors

Katerina Rozova
Iryna Mankovska

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