Vol 27, No 4 (2021)
Research paper
Published online: 2022-01-28
Characteristic of cells isolated from human Abdominal Aortic Aneurysm samples cultured in vitro
DOI: 10.5603/AA.2021.0014
Acta Angiologica 2021;27(4):120-129.
Abstract
Introduction: This study aimed to standardize cell culture methods for major cell types isolated from three
layers of human AAA. We also aimed to determine cell types in each layer of each AAA segment and compare
them with cell types in layers of control, unchanged segments.
Material and methods: We divided AAAs into three segments along the AAA and control segments flanking
the aneurysm. Isolated cells following expansion were analyzed by flow cytometry, immunochemistry, and
microscopic methods. Fluorochrome-conjugated antibodies were used to detect the three major cell types
(endothelial cells, smooth muscle cells, and fibroblasts) in each layer of every AAA segment.
Results: The culture of cells from the three AAA segments was successfully established in 21% of patients.
In all of the layers, only a small proportion of cells showed layer-specific markers of cell types. The majority of
cells from every layer were positive for CD90, which is considereda specific marker of fibroblasts in the aorta.
Conclusions: We describe a methodology for isolation of cells, their culture conditions, and phenotypic
characterization for AAA. The wall of AAA loses its specific types of cells in all of the layers compared with the
normal abdominal aortic wall.
Keywords: abdominal aortic aneurysmaortic adventitial fibroblastaortic endothelial cellsaorta smooth musclesAAA segmentation
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