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Characteristic of cells isolated from human Abdominal Aortic Aneurysm samples cultured in vitro
Affiliations- Medical University of Silesia, Department Of Molecular Biology, Medyków 18, C1/IV p., 40-752 Katowice, Poland
- Medical University of Silesia, Department of Medical Genetics, Medyków 18, C1/IV p., 40-752 Katowice, Poland
- Medical University of Silesia, Department of General Vascular Surgery, Ziołowa 45/47, 40-635 Katowice, Poland
- Department of Surgical Oncology and Vascular Surgery, Oncological Centre in Katowice, ul. Raciborska 26, 40-074 Katowice, Poland
open access
Abstract
Introduction: This study aimed to standardize cell culture methods for major cell types isolated from three
layers of human AAA. We also aimed to determine cell types in each layer of each AAA segment and compare
them with cell types in layers of control, unchanged segments.
Material and methods: We divided AAAs into three segments along the AAA and control segments flanking
the aneurysm. Isolated cells following expansion were analyzed by flow cytometry, immunochemistry, and
microscopic methods. Fluorochrome-conjugated antibodies were used to detect the three major cell types
(endothelial cells, smooth muscle cells, and fibroblasts) in each layer of every AAA segment.
Results: The culture of cells from the three AAA segments was successfully established in 21% of patients.
In all of the layers, only a small proportion of cells showed layer-specific markers of cell types. The majority of
cells from every layer were positive for CD90, which is considereda specific marker of fibroblasts in the aorta.
Conclusions: We describe a methodology for isolation of cells, their culture conditions, and phenotypic
characterization for AAA. The wall of AAA loses its specific types of cells in all of the layers compared with the
normal abdominal aortic wall.
Abstract
Introduction: This study aimed to standardize cell culture methods for major cell types isolated from three
layers of human AAA. We also aimed to determine cell types in each layer of each AAA segment and compare
them with cell types in layers of control, unchanged segments.
Material and methods: We divided AAAs into three segments along the AAA and control segments flanking
the aneurysm. Isolated cells following expansion were analyzed by flow cytometry, immunochemistry, and
microscopic methods. Fluorochrome-conjugated antibodies were used to detect the three major cell types
(endothelial cells, smooth muscle cells, and fibroblasts) in each layer of every AAA segment.
Results: The culture of cells from the three AAA segments was successfully established in 21% of patients.
In all of the layers, only a small proportion of cells showed layer-specific markers of cell types. The majority of
cells from every layer were positive for CD90, which is considereda specific marker of fibroblasts in the aorta.
Conclusions: We describe a methodology for isolation of cells, their culture conditions, and phenotypic
characterization for AAA. The wall of AAA loses its specific types of cells in all of the layers compared with the
normal abdominal aortic wall.
Keywords
abdominal aortic aneurysm, aortic adventitial fibroblast, aortic endothelial cells, aorta smooth muscles, AAA segmentation
About this articleTitle
Characteristic of cells isolated from human Abdominal Aortic Aneurysm samples cultured in vitro
Journal
Issue
Article type
Research paper
Pages
120-129
Published online
2022-01-28
Page views
4452
Article views/downloads
299
DOI
10.5603/AA.2021.0014
Bibliographic record
Acta Angiologica 2021;27(4):120-129.
Keywords
abdominal aortic aneurysm
aortic adventitial fibroblast
aortic endothelial cells
aorta smooth muscles
AAA segmentation
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